Sökning: "mRNA quantification"

Visar resultat 21 - 25 av 43 avhandlingar innehållade orden mRNA quantification.

  1. 21. Tissue Factor and CD40 Ligand : Markers for the Interplay of Coagulation and Inflammation in the Acute Coronary Syndrome

    Författare :Anders Mälarstig; Agneta Siegbahn; Lars Wallentin; Taavo Tenno; Fiona Green; Uppsala universitet; []
    Nyckelord :Molecular medicine; tissue factor; CD40 ligand; gene expression; single nucleotide polymorphism; acute coronary syndrome; myocardial infarction; coagulation; inflammation; Molekylärmedicin;

    Sammanfattning : BACKGROUND: Tissue factor (TF) is a 47 kDa transmembrane glycoprotein known as the main initiator of blood coagulation. CD40 ligand is another membrane molecule, which ligates to cell types associated with atherosclerotic plaques thereby mediating intraplaque inflammation and weakening of the fibrous cap. LÄS MER

  3. 22. Targeting the GH/IGF-1 axis with novel, small molecule inhibitors

    Författare :Linda Rosengren; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : The growth hormone (GH) / insulin-like growth factor (IGF) family of ligands, binding proteins and receptors play multiple roles in cell growth, metabolism and development. In addition, numerous studies have demonstrated the pathophysiological importance of the GH/IGF-1 axis. LÄS MER

  4. 23. Using Minisequencing Technology for Analysing Genetic Variation in DNA and RNA

    Författare :Mona Fredriksson; Ann-Christine Syvänen; Aarno Palotie; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; microarray; minisequencing; molecular medicine; single nucleotide polymorphism; stem cell transplantation; whole genome amplification; allelic imbalance; alternative splicing; Molekylärmedicin; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ;

    Sammanfattning : In this thesis, the four-color fluorescence tag-microarray minisequencing system pioneered by our group was further developed and applied for analysing genetic variation in human DNA and RNA. A SNP marker panel representing different chromosomal regions was established and used for identification of informative SNP markers for monitoring chimerism after stem cell transplantation (SCT). LÄS MER

  5. 24. Cytokine induced airway hyperreactivity characterized in an in-vitro model of asthma

    Författare :Yaping Zhang; Klinisk och experimentell allergiforskning; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; toxicology; Farmakologi; farmakognosi; MAPK; TNF-alpha; 5-HT; Respiratory system; Andningsorganen; Pharmacological sciences; pharmacognosy; pharmacy; interleukin-1beta; hyperresponsiveness; endothelin-1; airway; bradykinin; toxikologi; farmaci;

    Sammanfattning : Asthma is a chronic disorder of the lower airways, characterized by inflammation and hyperreactivity. Pro-inflammatory cytokines like tumour necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) are known to play important roles in the pathogenesis, but our knowledge how these cytokines contribute to the development of airway hyperreactivity is limited. LÄS MER

  7. 25. Effects of Biomechanical Stress on Gene Regulation in Vascular Cells

    Författare :Maria Carlström; Göteborgs universitet; []
    Nyckelord :shear stress; intraluminal pressure; endothelium; gene expression; DNA microarray; real-time RT-PCR; reference genes; smooth muscle cells; P2 receptors; TNF-alpha; urokinase-type plasminogen activator;

    Sammanfattning : The vascular vessel wall is constantly exposed to biomechanical forces, such as shear and tensile stress. Biomechanical forces are important for several physiological and pathological processes and have been shown to regulate a number of fundamental vascular functions, such as vascular tone and remodeling processes. LÄS MER