Sökning: "mRNA quantification"

Visar resultat 6 - 10 av 43 avhandlingar innehållade orden mRNA quantification.

  1. 6. Real-time PCR and its application for protein quantification

    Författare :Kristina Lind; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; ELISA; real-time immuno-PCR; real-time PCR; melt curve analysis; PSA; 3’UTR; TAP-tag; BOXTO; immuno-qPCR;

    Sammanfattning : The ability to quantify specific DNA sequences with real-time PCR has increased the possibilities for sensitive DNA analysis enormously. This sensitive technique can in optimal conditions detect one DNA copy and has a large quantification range. LÄS MER

  3. 7. Multiparametric Optical Characterization of Biological Nanoparticles using Evanescent Field Sensing

    Författare :Mattias Sjöberg; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein corona; single-particle; liposomes; SPR; lipid nanoparticles; Waveguide scattering microscopy; label-free; mass quantification.;

    Sammanfattning : In light of the increasingly realized dependence of many biological functions on nanoscopic supramolecular assemblies, also including novel biotechnological applications, there is a need for advanced analysis methods capable of accurately quantifying different characteristics of these elusive entities. The prime aim of this thesis is the development and utilization of surface-based bioanalytical sensing methods for quantitative characterization of biological nanoparticles. LÄS MER

  4. 8. Detection and quantification of lipid-mediated siRNA delivery to the cytosol

    Författare :Hampus Hedlund; Tumörmikromiljö; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; RNA Interference; RNA Therapeutics; Endosomal Escape; Lipid Mediated Delivery; Live-Cell Imaging;

    Sammanfattning : Cancer is one of the leading causes of death globally. Novel therapeutics is urgently needed to advance treatment, especially for generalized disease where current treatment options have a poor prognosis. In particular, targeted therapy that can address genetic changes would be of great value. LÄS MER

  5. 9. Real-time PCR for diagnosis of botulism and quantification of neurotoxin gene expression in Clostridium botulinum

    Författare :Ingrid Artin; Avdelningen för medicinsk mikrobiologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; detection; qRT-PCR; Clostridium botulinum; molecular methods;

    Sammanfattning : The objectives of the work presented in this thesis have been to develop molecular methods for (i) detection of Clostridium botulinum in food and clinical samples, and (ii) monitoring the expression of the neurotoxin gene, cnt, during different growth phases and under different growth conditions. For the diagnosis of botulism, a real-time PCR-based method including an internal amplification control for C. LÄS MER

  7. 10. At the intersection of proteomics and pathology : application of mass spectrometry-based protein quantification to histopathology and antibody validation

    Författare :Fabio Socciarelli; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : The paradigm of Precision Medicine relies on the molecular stratification of patients to select “the right patient for the right drug”. The genomic approach has tried to answer this question, but large genomic studies have raised more questions than given answers. LÄS MER