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Hittade 4 avhandlingar som matchar ovanstående sökkriterier.

  1. 1. Construction, expression and evaluation of anti-keratin 8 single-chain antibody fragments

    Författare :Rozbeh Jafari; Birgitta Sundström; Jörgen Carlsson; Karlstads universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Single-chain antibody fragments; keratin 8; site-directed mutagenesis; heterologous expression; P. pastoris; design of experiments; multicellular tumor spheroids; immunotargeting; Biomedical Sciences; Biomedicinsk vetenskap;

    Sammanfattning : Antibodies are glycoproteins specifically binding to a variety of antigens and today extensively used in prevention, diagnosis and treatment of diseases. Carcinomas originate from epithelial tissues and are the most common forms of human malignancies. In the necrotic areas of carcinomas significant amounts of keratins (K) are found extracellularly. LÄS MER

  3. 2. Single-chain antibody construction and functional mapping of the monoclonal antibody TS1 : Its interaction with the antigen and the anti-idiotype

    Författare :Patrik Holm; Birgitta Sundström; Per-Åke Nygren; Karlstads universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Chemistry; Kemi; Chemistry; Kemi; Biomedicin; Biomedical Laboratory Science;

    Sammanfattning : The aims of this study are to synthesize and produce a single-chain antibody (scFv) of the anti-cytokeratin 8 monoclonal IgG antibody TS1 and to functionally map amino acid residues important for the interaction with its antigen and the anti-idiotypic antibody TS1. The TS1 antibody has been shown to be effective in binding cytokeratin 8 (CK8) expressed in tumors in vivo and is proposed to be useful in immunotargeting and/or immunotherapy. LÄS MER

  4. 3. Towards Personalized Cancer Therapy : New Diagnostic Biomarkers and Radiosensitization Strategies

    Författare :Diana Spiegelberg; Marika Nestor; Bo Stenerlöw; Johan Lennartson; Theodosia Maina-Nock; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; tumor targeting; radionuclide targeting; HSP90 inhibition; AT13387; radiosensitization; molecular imaging; combination treatment; EGFR; CD44v6; Biomedical Radiation Science; Biomedicinsk strålningsvetenskap;

    Sammanfattning : This thesis focuses on the evaluation of biomarkers for radio-immunodiagnostics and radio-immunotherapy and on radiosensitization strategies after HSP90 inhibition, as a step towards more personalized cancer medicine. There is a need to develop new tracers that target cancer-specific biomarkers to improve diagnostic imaging, as well as to combine treatment strategies to potentiate synergistic effects. LÄS MER

  5. 4. Interaction studies of idiotypic and antiidiotypic antibodies at experimental tumor targeting

    Författare :Ann Erlandsson; Mats Ohlin; Umeå universitet; []
    Nyckelord :anti-idiotypic antibody; clearing; cytokeratin 8; epitope; idiotypic antibody; immunotherapy;

    Sammanfattning : An increasing number of antibodies are being labeled with radionuclides for immunotargeting, but the negative side effects generated on normal tissues by the nuclide remains a significant problem. The clearing of non-tumor-targeted radiolabeled antibodies from the circulation contributes significantly to improved targeting; it has been demonstrated that anti-idiotypic antibodies can be used for this purpose. LÄS MER