Sökning: "P. pastoris"

Visar resultat 1 - 5 av 21 avhandlingar innehållade orden P. pastoris.

  1. 1. Process techniques for production of recombinant proteins with Picha pastoris

    Författare :Mehmedalija Jahic; KTH; []
    Nyckelord :p. Pastoris; fed-batch culture; proteolysis; modeling; TECHNOLOGY; TEKNIKVETENSKAP;

    Sammanfattning : .... LÄS MER

  3. 2. Construction, expression and evaluation of anti-keratin 8 single-chain antibody fragments

    Författare :Rozbeh Jafari; Birgitta Sundström; Jörgen Carlsson; Karlstads universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Single-chain antibody fragments; keratin 8; site-directed mutagenesis; heterologous expression; P. pastoris; design of experiments; multicellular tumor spheroids; immunotargeting; Biomedical Sciences; Biomedicinsk vetenskap;

    Sammanfattning : Antibodies are glycoproteins specifically binding to a variety of antigens and today extensively used in prevention, diagnosis and treatment of diseases. Carcinomas originate from epithelial tissues and are the most common forms of human malignancies. In the necrotic areas of carcinomas significant amounts of keratins (K) are found extracellularly. LÄS MER

  4. 3. Exploring Unorthodox Aquaporins : Characterization of NbXIPs & AtNIPs

    Författare :Henry Ampah-Korsah; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Aquaporins; XIPs; NIPs; Phosphorylation; Pichia pastoris; Overexpression; boric acid; membrane proteins;

    Sammanfattning : AbstractAquaporins are membrane integral proteins that facilitate the transport of waterand/or other small neutral molecules across biological membranes in cells in allforms of life. Among the subfamilies of aquaporins in higher plants, the X IntrinsicProteins (XIPs) are the most recently discovered subfamily and the leastcharacterized. LÄS MER

  5. 4. Expression and Function of Human and Plant Aquaporins

    Författare :Maria Agemark; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; aquaporin; water channel; overexpression; Pichia pastoris; AQP8; AQP5; SoPIP2; 1;

    Sammanfattning : The aquaporins (AQPs) belong to a family of water permeable membrane channels found in virtually all living organisms. Thirteen isoforms of mammalian AQPs are found, whereas in Arabidopsis thaliana 35 genes encoding AQPs are found. The AQPs are distributed in different organs, cell types and in different subcellular membranes. LÄS MER

  7. 5. Expression of a lipase in prokaryote and eukaryote host systems allowing engineering

    Författare :Marianne Wittrup Larsen; Karl Hult; Marina Lotti; KTH; []
    Nyckelord :pseudozzyma candida antarctica lipase B; escherichia coli; pichia pastoris; rational design;

    Sammanfattning : Pseudozyma (Candida) antarctica lipase B (PalB) was expressed in Escherichia coli facilitating protein engineering. The lack of glycosylation was evaluated for a deeper understanding of the difficulties in expressing PalB in E. coli. LÄS MER