Sökning: "fluorescent labelling"

Visar resultat 1 - 5 av 20 avhandlingar innehållade orden fluorescent labelling.

1. 1. Nano- and Micro-sized Molecularly Imprinted Polymer Materials for Analytical Application

   Författare :Keiichi Yoshimatsu; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; spin-coating; solid-phase extraction; composite; electrospinning; precipitation polymerisation; Enkephalin; fluorescent labelling; propranolol; nanoparticles; scintillation proximity assay; Molecular imprinting; Molecularly imprinted polymers; nanofibers; thin-film; microspheres;

   Sammanfattning : Specific molecular recognition materials are important in analytical applications. It has been a longstanding dream for scientific explorers to realise a versatile method to generate materials that can recognize biological and chemical substance of interests. LÄS MER

3. 2. Novel fluorescent tools and techniques for 3D imaging of the cleared brain

   Författare :Alex Yu Hin Wong; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Background: To better understand the complexity of the brain and how it becomes impaired under different pathological states, a considerably large number of brains would be needed for imaging to generate highly detailed maps in 3D. Chemical probes can offer a readily scalable labelling method that is robust, easy to use with the quick operation, and feasible for human tissue where genetic viral and toxin tracers are inappropriate. LÄS MER

4. 3. Localisation of Fluorescent Probes and the estimation of Lipid Nanodomain sizes by modern fluorescence techniques

   Författare :Radek Sachl; Lennart B-Å Johansson; Martin Hof; Mats Almgren; Tomas Obsil; Umeå universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Electronic energy transfer; fluorescence; rafts; lipid bilayer; bicelles; micelles; fysikalisk kemi; Physical Chemistry;

   Sammanfattning : The thesis is divided into two major parts. The first part focuses on the localisation of probes in lipid/polymeric bilayers and in GM1 micelles. Included in this thesis is a new approach based on electronic energy transfer/migration (FRET/DDEM), which efficiently determines transversal positions of fluorescent molecules in lipid bilayers. LÄS MER

5. 4. 3D super-resolution microscopy of living cells using reversibly switchable fluorophores

   Författare :Andreas Bodén; Ilaria Testa; Francisco Balzarotti; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; RESOLFT; 3D imaging; super-resolution; microscopy; reversibly switchable fluorescent proteins; Biological Physics; Biologisk fysik;

   Sammanfattning : Traditional optical microscopy techniques are limited in spatial resolution due to the wave nature of light. This means that neighboring objects separated by a distance smaller than about 200 nm cannot be distinguished. LÄS MER

7. 5. In vivo properties of neural stem cells after transplantation into the rat brain-Studies of phenotypic differentiation and functional integration using cell-specific labelling and electrophysiological techniques

   Författare :Ulrica Englund Johansson; Peter Eriksson; Lund Oftalmologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; cell line; Neurology; neuropsychology; neurophysiology; Neurologi; neuropsykologi; neurofysiologi; functional integration; electrophysiology; retrograde tracing; immortalized; GFP; migration; axonal projections; glia; neuron; transplantation; stem cell. progenitor cell; CNS; Biomedical Sciences;

   Sammanfattning : In the present thesis, we have examined the in vivo properties of in vitro expanded human and rat neural stem-and progenitor cells after transplantation into the neonatal and adult rat brain. The survival and differentiation of the grafted cells were assessed using species-specific antisera, and pre-labelling with the reporter gene green fluorescent protein (GFP). LÄS MER