Sökning: "Ulf Landegren"

Visar resultat 6 - 10 av 33 avhandlingar innehållade orden Ulf Landegren.

1. 6. Selector Technology : For Multiplex DNA Analysis

   Författare :Fredrik Dahl; Mats Nilsson; Ulf Landegren; Ivo Glynne Gut; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Genetic engineering; DNA technology; Genteknik; Genetic engineering including functional genomics; Genteknik inkl. funktionsgenomik;

   Sammanfattning : A majority of methods for identifying sequences in the human genome involve target sequence amplification through PCR. This work presents novel methods for amplifying circularized DNA and presents solutions for some major limitations of PCR. LÄS MER

3. 7. Solid-phase Proximity Ligation Assays : High-performance and multiplex protein analyses

   Författare :Spyros Darmanis; Masood Kamali-Moghaddam; Ulf Landegren; Mathias Uhlen; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; proximity ligation assay; multiplexed; biomarkers; solid-phase; microparticles; cancer; sequencing; Molecular biology; Molekylärbiologi; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

   Sammanfattning : Protein biomarkers circulating in blood hold the promise of improved diagnosis, prognosis and follow-up of treatment of disease via minimally invasive procedures. For the discovery and validation of such biomarkers, methods are needed that can facilitate parallel, highly specific and in-depth analysis of the blood proteome. LÄS MER

4. 8. Development and Application of Proximity Assays for Proteome Analysis in Medicine

   Författare :Felipe Marques Souza de Oliveira; Masood Kamali-Moghaddam; Ulf Landegren; Mats Gullberg; Janne Lehtiö; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Solid-phase proximity ligation assay; post-translational modifications; glycosylation; phosphorylation; Enzyme-linked immunosorbent assay; immunoassay and rolling circle amplification; Proximity Extension Assay; inflammation protein biomarkers; autoantibodies; autoimmune disease;

   Sammanfattning : Along with proteins, a myriad of different molecular biomarkers, such as post-translational modifications and autoantibodies, could be used in an attempt to improve disease detection and progression. In this thesis, I build on several iterations of the proximity ligation assay to develop and apply new adaptable methods to facilitate detection of proteins, autoantibodies and post-translational modifications. LÄS MER

5. 9. Barcoded DNA Sequencing for Parallel Protein Detection

   Författare :Mahya Dezfouli; Afshin Ahmadian; Ulf Landegren; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; DNA barcoding; antibody labeling; antibody oligonucleotide bio-conjugation; DNAassisted proteomics; immuno-sequencing I-Seq ; droplet-based system; large-scale data analysis; Biotechnology; Bioteknologi;

   Sammanfattning : The work presented in this thesis describes methodologies developed for integration and accurate interpretation of barcoded DNA, to empower large-scale-omics analysis. The objectives mainly aim at enabling multiplexed proteomic measurements in high-throughput format through DNA barcoding and massive parallel sequencing. LÄS MER

7. 10. Development of Enhanced Molecular Diagnostic Tools for Protein Detection and Analysis

   Författare :Tonge Ebai; Ulf Landegren; Masood Kamali-Moghaddam; Peter Nilsson; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; protein detection; proximity ligation assays; proximity extension assay; rolling circle amplification; ELISA; flow cytometry; fluorescence microscopy;

   Sammanfattning : Improved diagnosis, prognosis and disease follow-up is a fundamental procedure and a constant challenge in medicine.  Among the different molecular biomarkers, proteins are the essential regulatory component in blood; hence, by developing enhanced specific and sensitive molecular tools will gives great insight into the different processes in disease treatment. LÄS MER