Sökning: "Ulf Landegren"
Visar resultat 16 - 20 av 33 avhandlingar innehållade orden Ulf Landegren.
16. Application of Padlock Probe Based Nucleic Acid Analysis In Situ
Sammanfattning : The great variation displayed by nucleic acid molecules in human cells, and the continuous discovery of their impact on life, consequently require continuous refinements of molecular analysis techniques. Padlock probes and rolling circle amplification offer single nucleotide discrimination in situ, a high signal-to-noise ratio and localized detection within cells and tissues. LÄS MER
17. DNA Tools and Microfluidic Systems for Molecular Analysis
Sammanfattning : Improved methods are needed to interrogate the genome and the proteome. Methods with high selectivity, wide dynamic range, and excellent precision, capable of simultaneously analyzing many biomolecules are required to decipher cellular function. This thesis describes a molecular and microfluidic toolbox designed with those criteria in mind. LÄS MER
18. Visualization of Protein Activity Status in situ Using Proximity Ligation Assays
Sammanfattning : In 2001 the human proteome organization (HUPO) was created with the ambition to identify and characterize all proteins encoded in the human genome according to several criteria; their expression levels in different tissues and under different conditions; the sub-cellular localization; post-translational modifications; interactions, and if possible also the relationship between their structure and function.When the knowledge of different proteins and their potential interactions increases, so does the need for methods able to unravel the nature of molecular processes in cells and organized tissues, and ultimately for clinical use in samples obtained from patients. LÄS MER
19. Detection and Sequencing of Amplified Single Molecules
Sammanfattning : Improved analytical methods provide new opportunities for both biological research and medical applications. This thesis describes several novel molecular techniques for nucleic acid and protein analysis based on detection or sequencing of amplified single molecules (ASMs). LÄS MER
20. Single Molecule Detection : Microfluidic Automation and Digital Quantification
Sammanfattning : Much of recent progress in medical research and diagnostics has been enabled through the advances in molecular analysis technologies, which now permit the detection and analysis of single molecules with high sensitivity and specificity. Assay sensitivity is fundamentally limited by the efficiency of the detection method used for read-out. LÄS MER
