Sökning: "Klenow DNA polymerase"
Visar resultat 1 - 5 av 6 avhandlingar innehållade orden Klenow DNA polymerase.
1. Mathematical modelling and analysis of the pyrosequencing reaction system
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2. Protein Engineering by Directed Evolution and Rational Design
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3. Recombinant Enzymes in Pyrosequencing Technology
Sammanfattning : Pyrosequencing is a DNA sequencing method based on thedetection of released pyrophosphate (PPi) during DNA synthesis.In a cascade of enzymatic reactions, visible light isgenerated, which is proportional to the number of nucleotidesincorporated into the DNA template. When dNTP(s) areincorporated into the DNA template, inorganic PPi is released. LÄS MER
4. Advancements in Firefly Luciferase-Based Assays and Pyrosequencing Technology
Sammanfattning : Pyrosequencing is a new DNA sequencing method relying on thesequencing-by-synthesis principle and bioluminometric detectionof nucleotide incorporation events. The objective of thisthesis was improvement of the Pyrosequencing method byincreasing the thermal stability of firefly luciferase, and byintroducing an alternative DNA polymerase and a new nucleotideanalog. LÄS MER
5. Effects of fusion tags on protein partitioning In aqueous two-phase systems and use in primary protein recovery
Sammanfattning : The two techniques aqueoustwo-phase partitioning and expanded bed adsorption that bothare suitable for primary protein recovery were studied. Most ofthe work was focused on partition in aqueous two-phase systemsand in particular on the possibility to effect the partitionbehaviour by fusion of short peptide tags or protein domains tothe target protein. LÄS MER