Sökning: "single cell tracking"

Visar resultat 1 - 5 av 29 avhandlingar innehållade orden single cell tracking.

  1. 1. Live Single Cell Imaging and Analysis Using Microfluidic Devices

    Författare :Mohammad Ali Khorshidi; Helene Andersson-Svahn; Håkan Jönsson; Carolina Wählby; Johan Elf; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Single cell analysis; time-lapse fluorescence imaging; automated image analysis; microwell; droplet microfluidics; NK cells; single cell tracking; migration behavior analysis; cell-cell interaction; optical microscopy; image analysis; image processing; microfluidics; immune cells; tracking; counting; morphology analysis;

    Sammanfattning : Today many cell biological techniques study large cell populations where an average estimate of individual cells’ behavior is observed. On the other hand, single cell analysis is required for studying functional heterogeneities between cells within populations. LÄS MER

  3. 2. Tracking single molecules in uncharted territory : A single-molecule method to study kinetics in live bacteria

    Författare :Javier Aguirre Rivera; Magnus Johansson; Dmitri N. Ermolenko; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; single-molecule; protein synthesis; fluorescence microscopy; antibiotics; aminoglycosides; macrolides; apramycin; gentamicin; paromomycin; S13; translation; bacteria; Molekylär biovetenskap; Molecular Life Sciences;

    Sammanfattning : The synthesis of proteins, also known as translation, is a fundamental process in every living organism. The steps in the translation of genetic information to functional proteins have been meticulously studied, mostly using in vitro techniques, yielding a detailed model of their mechanisms. LÄS MER

  4. 3. Microfluidics and AI for single-cell microbiology

    Författare :Praneeth Karempudi; Johan Elf; Yoav Shechtman; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Microfluidics; Artificial intelligence; Deep learning; Single-cell microbiology; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

    Sammanfattning : Most of the biological sciences deal with understanding the relationships between phenotypes and the underlying molecular mechanisms of organisms. This thesis is an engineering, computational, and experimental exercise in expanding the scope and scale of phenotype-genotype mapping techniques in single-cell microbiology using microscopy, microfluidics, and image processing. LÄS MER

  5. 4. Biological Insights from Single-Particle Tracking in Living Cells

    Författare :Arash Sanamrad; Johan Elf; Måns Ehrenberg; Achillefs Kapanidis; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; single-particle tracking; intracellular diffusion; nucleoid exclusion; transcription-translation coupling; antibiotics; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

    Sammanfattning : Single-particle tracking is a technique that allows for quantitative analysis of the localization and movement of particles. In this technique, trajectories are constructed by determining and connecting the positions of individual particles from consecutive images. LÄS MER

  7. 5. Pathogenic Neisseria : Single cell motility, multicellular dynamics and antimicrobial susceptibility

    Författare :Olaspers Sara Eriksson; Ann-Beth Jonsson; Matthew Francis; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Neisseria; Type IV pili; Twitching motility; PilT; Microcolony dynamics; PilU; NafA; Antimicrobial peptide; Cell-penetrating peptide; molekylär biovetenskap; Molecular Bioscience;

    Sammanfattning : Neisseria meningitidis and Neisseria gonorrhoeae can colonize humans without causing any symptoms. However, gonorrhea and invasive meningococcal disease are serious health concerns. An essential virulence factor for neisserial adhesion to host cells, twitching motility and microcolony formation/aggregation is the retractile type IV pili (Tfp). LÄS MER