Sökning: "Single Nucleotide"

Visar resultat 11 - 15 av 437 avhandlingar innehållade orden Single Nucleotide.

1. 11. Förster Resonance Energy Transfer - from single molecule spectroscopy to imaging

   Författare :Evangelos Sisamakis; Jerker Widengren; Paul R Selvin; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; FRET; FCS; single-molecule biophysics;

   Sammanfattning : During the last fifteen years several methods have been developed for probing biomolecules (DNA, RNA, proteins) one at a time. Among these methods fluorescence spectroscopy and in particular its many implementations for monitoring Förster Resonance Energy Transfer (FRET), have attracted much interest. LÄS MER

3. 12. Detecting Sex and Selection in Ancient Cattle Remains Using Single Nucleotide Polymorphisms

   Författare :Emma M Svensson; Anders Götherström; Juha Kantanen; Uppsala universitet; []
   Nyckelord :HUMANIORA; HUMANITIES; NATURVETENSKAP; NATURAL SCIENCES; aurochs; cattle; ancient DNA; SNP; pyrosequencing; selection; Bos taurus; Historical osteology; Historisk osteologi; Genetics; Genetik; Biology with specialization in Evolutionary Genetics; Biologi med inriktning mot evolutionär genetik;

   Sammanfattning : All contemporary taurine cattle originated some 10,000 years ago when their wild ancestor, the aurochs, was domesticated in the Near East. Although the aurochs was widespread also in Europe, there is no evidence for a local domestication. The aurochs has been extinct since 1627 and therefore little is known about its biology. LÄS MER

4. 13. Functional analysis of the -1087 single nucleotide polymorphism in the IL-10 promoter region

   Författare :Lena Larsson; Göteborgs universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; IL-10; B cells; Sp1; transcription factors; gene expression;

   Sammanfattning : Interleukin (IL) 10 is recognized as a pro-inflammatory cytokine that promotes B cell proliferation. The objectives of the present series of studies were to analyze (i) differences in transcription factor binding to the -1087 IL-10 gene polymorphism in B cells, (ii) the influence of the A to G nucleotide transition on IL-10 and Sp1 gene expression in B cells, (iii) differences in Sp1 expression in periodontitis lesions from GG and AA genotype subjects and (iv) epigenetic modifications around the -1087 site and their influence on IL-10 gene expression. LÄS MER

5. 14. Search for Type 2 Diabetes Susceptibility Genes Using Multiple Approaches

   Författare :Cecilia Lindgren; Malmö Institutionen för kliniska vetenskaper; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; single nucleotide polymorphism SNP ; transmission-disequilibrium test TDT ; genetic variation; single strand conformational polymorphism SSCP ; gene expression; insulin; glucose; genome-wide scan; linkage; genetic association; Type 2 diabetes; early onset diabetes EOD ; sekretion; diabetologi; Endokrinologi; diabetology; Endocrinology; secreting systems; signal-to-noise ratio SNR ;

   Sammanfattning : Type 2 diabetes mellitus is a multifactorial disorder characterized by chronic hyperglycemia resulting from pancreatic dysfunction and insulin resistance. It is a common disorder with a complex pattern of inheritance, likely to reflect the influence of multiple genetic and environmental factors on the diabetes risk. LÄS MER

7. 15. Applications of Four-Colour Fluorescent Primer Extension Technology for SNP Analysis and Discovery

   Författare :Annika Ahlford; Ann-Christine Syvänen; Mats Nilsson; David Schwartz; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Single nucleotide polymorphism; Genotyping; Massively parallel sequencing; Gene mapping; Lab-on-a-chip; Medical genetics; Medicinsk genetik; Molecular biology; Molekylärbiologi; Molekylär medicin; Molecular Medicine;

   Sammanfattning : Studies on genetic variation can reveal effects on traits and disease, both in humans and in model organisms. Good technology for the analysis of DNA sequence variations is critical. Currently the development towards assays for large-scale and parallel DNA sequencing and genotyping is progressing rapidly. LÄS MER