Sökning: "DNA microarray"

Visar resultat 1 - 5 av 173 avhandlingar innehållade orden DNA microarray.

  1. 1. Protein Microarray Chips

    Författare :Goran Klenkar; Bo Liedberg; Brian MacCraith; Linköpings universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Microarray; biosensor; biomolecular interaction; narcotics detection; imaging ellipsometry and surface plasmon resonance; Molecular biophysics; Molekylär biofysik;

    Sammanfattning : Livet tas för givet av de flesta. Det finns däremot många som ägnar stora delar av sitt liv för att försöka lösa dess mysterier. En del av lösningen ligger i att förstå hur alla molekyler är sammanlänkade i det gigantiska nätverk som definierar den levande organismen. LÄS MER

  3. 2. Arrayed identification of DNA signatures

    Författare :Max Käller; Lundeberg Joakim; Mats Nilsson; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; apyrase; allele-specific extension; competitive hybridization; DNA sequencing; genotyping; human papillomavirus HPV ; MC1R; microarray; mutation; p53; protease; Bioengineering; Bioteknik;

    Sammanfattning : In this thesis techniques are presented that aim to determine individual DNA signatures by controlled synthesis of nucleic acid multimers. Allele-specific extension reactions with an improved specificity were applied for several genomic purposes. Since DNA polymerases extend some mismatched 3’-end primers, an improved specificity is a concern. LÄS MER

  4. 3. Weighted Analysis of Microarray Experiments

    Författare :Anders Sjögren; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; DNA microarray; differential expression; gene expression; quality control QC ; quality assurance QA ; quality assessment; generalised linear model; empirical Bayes; weighted moderated statistic; invalid p-value; heteroscedasticity; WAME; heteroscedasticity;

    Sammanfattning : DNA microarrays are strikingly efficient tools for analysing gene expression for large sets of genes simultaneously. The aim is often to identify genes which are differentially expressed between some studied conditions, thereby gaining insight into which cellular mechanisms are differently active between the conditions. LÄS MER

  5. 4. Microarray Technology for Genotyping in Pharmacogenetics

    Författare :Ulrika Liljedahl; Ann-Christine Syvänen; Denis Grant; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; microarray; genotyping; pharmacogenetics; molecular medicine; single nucleotide polymorphism; hypertension; Molekylärmedicin; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ;

    Sammanfattning : The studies in this thesis describe the development of a microarray based minisequencing system and its application to highly parallel genotyping of single nucleotide polymorphisms. The technical developments included identification of a three-dimensional microarray surface coating with high binding capacity for oligonucleotides modified with amino groups as the most optimal one for the system. LÄS MER

  7. 5. Genetic Sequence Analysis by Microarray Technology

    Författare :Emilie Hultin; Joakim Lundeberg; Afshin Ahmadian; Marie Allen; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Genotyping; single nucleotide polymorphism SNP ; protease-mediated allele-specific extension PrASE ; microarray; tag-array; competitive hybridization; human papillomavirus HPV ; single cell; loss of alleles; differentiation; epidermis.; Bioengineering; Bioteknik;

    Sammanfattning : Developments within the field of genetic analysis have during the last decade become enormous. Advances in DNA sequencing technology have increased throughput from a thousand bases to over a billion bases in a day and decreased the cost thousandfold per base. LÄS MER