Sökning: "Neutron protein crystallography"
Visar resultat 1 - 5 av 9 avhandlingar innehållade orden Neutron protein crystallography.
1. Computational protein crystallography : How to get the most out of your data
Sammanfattning : It is important to obtain accurate three dimensional structures of molecules and proteins to understand and predict their function and behaviour. X-ray crystallography is the major technique to determine three dimensional structures of proteins. LÄS MER
2. Production of Perdeuterated Proteins in Escherichia coli for Structural Studies by Neutron Crystallography
Sammanfattning : Neutron macromolecular crystallography (NMX) is a complementary technique to X-ray crystallography leveraging neutron scattering properties of the hydrogen isotope deuterium (2H). Production of deuterium labelled molecules together with large crystal requirements for neutron crystallography are few of several bottlenecks limiting implementation of this technique for a wider range of proteins. LÄS MER
3. Bridging the gap between computational chemistry and macromolecular crystallography
Sammanfattning : Knowledge of the atomic structure of biomolecules, such as proteins, is paramount to understanding their function and interactions in the human body. For example, knowledge of the atomic structure of a target protein is crucial for developing drugs that bind strongly to it and thus help cure diverse diseases. LÄS MER
4. Regulation of glycerol efflux in adipocytes. Structural and functional studies of the glycerol channel aquaporin 7
Sammanfattning : Glycerol levels in adipocytes depend on the lipolysis, the hydrolysis of triglycerides into glycerol and free fatty acids, and the efflux of glycerol across the plasma membrane through glycerol channels. The aims of this work were to investigate how glycerol levels are regulated on a molecular level by PLIN1, a major lipid droplet-associated protein and a key regulator of the lipolysis by scaffolding for lipolytic proteins on the lipid droplet. LÄS MER
5. Molecular recognition and dynamics in proteins studied by NMR
Sammanfattning : Knowledge of dynamics in protein is very important in the description of protein function and molecular recognition. The thesis investigates protein dynamics on time-scales from milli- to sub-nanosecond, with focus on the latter, using NMR spin relaxation experiments on two proteins, the 138-residue carbohydrate recognition domain of galectin-3 (Gal3C) and the 56-residue B1 domain of bacterial protein G (PGB1). LÄS MER