Sökning: "Perdeuteration"

Hittade 2 avhandlingar innehållade ordet Perdeuteration.

1. 1. Production of Perdeuterated Proteins in Escherichia coli for Structural Studies by Neutron Crystallography

   Författare :Vinardas Kelpsas; Molekylär cellbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Perdeuteration; Escherichia coli; triose-phosphate isomerase; Neutron crystallography; crystals;

   Sammanfattning : Neutron macromolecular crystallography (NMX) is a complementary technique to X-ray crystallography leveraging neutron scattering properties of the hydrogen isotope deuterium (2H). Production of deuterium labelled molecules together with large crystal requirements for neutron crystallography are few of several bottlenecks limiting implementation of this technique for a wider range of proteins. LÄS MER

3. 2. Perdeuteration of Biological Macromolecules: A Case Study of Human Carbonic Anhydrases

   Författare :Katarina Koruza; Molekylär cellbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Neutron protein crystallography; deuteration; protein production; cancer; inhibitor; human carbonic anhydrase IX;

   Sammanfattning : Deuterated biomolecules - where hydrogen atoms (H) are exchanged to its isotope deuterium (D) - are essential for biological experiments in neutron scattering, but they are not readily available. In neutron scattering, D has a strong positive coherent scattering length (6.67 fm) in comparison with the negative scattering length of H (-3.74 fm). LÄS MER