Sökning: "Feed rate"

Visar resultat 1 - 5 av 193 avhandlingar innehållade orden Feed rate.

  1. 1. Impact of glucose feed rate on productivity and recombinant protein quality in Escherichia coli

    Författare :Anna Maria Sandén; Gen Larsson; Gunnar Lidén; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Escherichia coli; recombinant protein production; fed-batch; feed profile; specific growth rate; Biokemi; Biochemistry; Biokemi;

    Sammanfattning : The goal of this work was to contribute to the fed-batch process optimisation task by deriving parameters that have considerable impact on productivity as well as product quality The chosen parameters were I) the design of the glucose feed profile, II) the choice of induction strategy, with respect to the method of addition, and III) the time of the induction, with respect to the specific glucose consumption rate.The present fed-batch experiments using the lacUV5-promoter, for production of b-galactosidase, have shown that a high glucose feed rate gives a specific production rate, qp, that is twice as high, after induction, compared to a feed rate that is 2. LÄS MER

  3. 2. Impact of glucose uptake rate on recombinant protein production in Escherichia coli

    Författare :Emma Bäcklund; Gen Larsson; Olle Holst; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; AIDA-autotransporter; Escherichia coli; fed-batch; glucose uptake rate; integral membrane proteins; outer membrane proteins; periplasmic retention; phosphotransferase system; recombinant proteins; specific growth rate; surface expression; Biology; Biologi;

    Sammanfattning : Escherichia coli (E. coli) is an attractive host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve high product quantities. LÄS MER

  4. 3. Integration of filamentous fungi in ethanol dry-mill biorefinery

    Författare :Jorge Ferreira; Högskolan i Borås; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; airlift bioreactors; ascomycetes; biomass; bubble column; ethanol; feed; Neurospora intermedia; thin stillage; zygomycetes; Resursåtervinning; Resource Recovery;

    Sammanfattning : The industrial production of bioethanol as a replacement to gasoline is well-established worldwide, using starch- or sugar-rich substrates. Additionally, the bioethanol plants produce animal feeds derived from fermentation leftovers. The biorefinery character of bioethanol plants can be enhanced via process diversification. LÄS MER

  5. 4. Growth rate control of periplasmic product retention in Escherichia coli

    Författare :Emma Bäcklund; Gen Larsson; Stefan Schmidt; Amelie Eriksson Karlström; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Escherichia coli; fed-batch; outer membrane proteins; recombinant proteins; specific growth rate; periplasmic retention; phosphotransferase system; high cell density cultivation; acetate formation; Bioengineering; Bioteknik;

    Sammanfattning : The recombinant product is secreted to the periplasm in many processes where E. coli is used as host. One drawback with secretion is the undesired leakage of the periplasmic products to the medium. The aim of this work was to find strategies to influence the periplasmic retention of recombinant products. LÄS MER

  7. 5. Microstructure model for Ti-6Al-4V used in simulation of additive manufacturing

    Författare :Corinne Charles Murgau; Lars-Erik Lindgren; Niklas Järvstråt; Robert Pederson; Sören Sjöström; Högskolan Väst; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Titanium alloy; Ti-6Al-4V; Welding; Metal deposition; Additive manufacturing; Wire feed; Finite Element Method; Microstructure model; Johnson-Mehl-Avrami- Kolmogorov; Thermally driven; Production Technology; Produktionsteknik; Manufacturing and materials engineering; Produktions- och materialteknik; Material Mechanics;

    Sammanfattning : This thesis is devoted to microstructure modelling of Ti-6Al-4V. The microstructure and the mechanical properties of titanium alloys are highly dependent on the temperature history experienced by the material. The developed microstructure model accounts for thermaldriving forces and is applicable for general temperature histories. LÄS MER