Sökning: "high cell density cultivation"

Visar resultat 1 - 5 av 12 avhandlingar innehållade orden high cell density cultivation.

  1. 1. Production of Ectoines and Poly(3-Hydroxybutyrate): High Cell-Density Cultivation of Halomonas Boliviensis

    Författare :Hector Guzmán; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; poly 3-hydroxybutyrate ; hydroxyectoine; ectoine; Halomonas boliviensis; compatible solutes; Fed-batch cultivation;

    Sammanfattning : This thesis reports a study involving the use of Halomonas boliviensis, a moderate halophile, for production of biopolyester poly(3-hydroxybutyrate) (PHB) and compatible solutes ectoine and hydroxyectoine through high cell-density cultivation comprising fed-batch cultures. PHB is a biodegradable plastic produced by bacteria as energy reserve and can potentially be used as a replacement of fossil based plastic. LÄS MER

  3. 2. High Cell Density Strategies and Adaptive Evolution for Microbial Propionic Acid Production

    Författare :Victor Hugo Cavero Olguin; Bioteknik; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : Propionic acid (PA) is a naturally occurring short chain fatty acid produced by microbial fermentation of the indigestible foods in the intestine, having a positive effect on the gastrointestinal health. PA inhibits the growth of pathogenic bacteria and molds; hence it is widely used as preservative for food, feed and grains. LÄS MER

  4. 3. Rhodothermus marinus: a cell factory : Exploring products and cultivation technology

    Författare :Emanuel Ron; Bioteknik; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Rhodothermus marinus; Extremophiles; Carotenoids; Exopolysaccharides;

    Sammanfattning : The unsustainable human impact on ecology and ensuing climate change necessitates the reduction of greenhouse gas emissions. The largest contributing industrial sector is the chemical industry, due to its petrochemical dependency. LÄS MER

  5. 4. Growth rate control of periplasmic product retention in Escherichia coli

    Författare :Emma Bäcklund; Gen Larsson; Stefan Schmidt; Amelie Eriksson Karlström; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Escherichia coli; fed-batch; outer membrane proteins; recombinant proteins; specific growth rate; periplasmic retention; phosphotransferase system; high cell density cultivation; acetate formation; Bioengineering; Bioteknik;

    Sammanfattning : The recombinant product is secreted to the periplasm in many processes where E. coli is used as host. One drawback with secretion is the undesired leakage of the periplasmic products to the medium. The aim of this work was to find strategies to influence the periplasmic retention of recombinant products. LÄS MER

  7. 5. Physiology of Saccharomyces cerevisiae Producing Recombinant Insulin in Continuous Culture

    Författare :Ali Kazemi Seresht; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; unfolded protein response; Keywords: heterologous protein production; prolonged chemostat; transcriptome; insulin analogue precursor; metabolic adaptation; URA3d; plasmid copy number; promoter truncation; high cell density cultivation; TPI1;

    Sammanfattning : The yeast Saccharomyces cerevisiae has widely been used as a host for the production of heterologous proteins. High-level production of heterologous proteins is likely to impose a metabolic burden on the host cell and can thus affect various aspects of cellular physiology. LÄS MER