Sökning: "FRET imaging"

Visar resultat 6 - 10 av 19 avhandlingar innehållade orden FRET imaging.

  1. 6. Fluorescence fluctuation studies of biomolecular interactions in solutions, biomembranes and live cells

    Författare :Volodymyr Chmyrov; Jerker Widengren; Antoine Delon; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; FCS; FCCS; Isomerization; TRAST; NMR; FRET; biomombrane; fluidity; Biological Physics; Biologisk fysik;

    Sammanfattning : Fluorescence spectroscopy and imaging have a very broad spectrum of applicationswithin the life sciences, in particular for detection and characterization ofbiomolecular dynamics and interactions in different environments. This thesis comprisesprojects that strive to further expand the information content extracted fromthe detected fluorescence, leading to sensitive readout parameters for studies ofbiomolecular dynamics and interactions. LÄS MER

  3. 7. Chemical Synthesis of Affibody Molecules for Protein Detection and Molecular Imaging

    Författare :Torun Ekblad; Amelie Eriksson Karlström; Per-Åke Nygren; Harald Kolmar; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Affibody; peptide synthesis; protein detection; molecular imaging; Bioengineering; Bioteknik;

    Sammanfattning : Proteins are essential components in most processes in living organisms. The detection and quantification of specific proteins can be used e.g. as measures of certain physiological conditions, and are therefore of great importance. LÄS MER

  4. 8. Modeling Biochemical Network Involved in Striatal Dopamine Signaling

    Författare :Anu G. Nair; Jeanette Hellgren Kotaleski; Omar Gutierrez Arenas; Upinder Singh Bhalla; Jakob Kisbye Dreyer; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Dopamine signaling; Striatum; Reward learning; Acetylcholine; Mass-action modeling; FRET biosensor; Adenosine; Eligibility trace; Computer Science; Datalogi;

    Sammanfattning : In this thesis, I studied the molecular integration of reward-learning related neuromodulatory inputs by striatal medium-sized projection neurons (MSNs) using mass-action kinetic modeling.It is known that, in reward learning, an unexpected reward results in transient elevation in dopamine (peak) whereas omission of an expected reward leads to transient dopamine decrease (dip). LÄS MER

  5. 9. Signal transduction via ion fluxes : a cell imaging study with emphasis on calcium oscillations

    Författare :Per Uhlén; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Calcium; Ca2 signalling; Ca2 oscillation; InsP3; InsP3 receptor; Na; K-ATPase; ouabain; physical interaction; FRET; spectral analysis; confocal microscopy;

    Sammanfattning : Signal transduction via ion fluxes is of vital importance for all mammalian cells. Cells can exploit ion regulatory mechanisms to generate and transmit intracellular signals. Improvements in digital microscopic imaging technology have made it possible to explore micro-environments and complex intracellular machineries. LÄS MER

  7. 10. Orientational dynamics of single molecules applied to protein-DNA target search

    Författare :Emil Marklund; Johan Elf; Erwin Peterman; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; single molecule imaging; fluorescence correlation spectroscopy; gene regulation; transcription factor; lac operon;

    Sammanfattning : Cells in all life forms regulate their genes with the help of transcription factors (TFs). These proteins turn genes on or off by binding regulatory motifs present in the DNA. Before this binding can occur, a TF needs to search and find the specific binding site in the genome of the cell. LÄS MER