Sökning: "FRET biosensor"
Hittade 5 avhandlingar innehållade orden FRET biosensor.
1. Fluorescence-based ligand assays for protein detection using affibody affinity proteins
Sammanfattning : The detection and quantification of biomolecules, and proteins in particular, are of great interest since these molecules are of fundamental importance to our well-being. Body fluids, as for instance human blood, are well suited for sampling of protein levels. LÄS MER
2. Modeling Biochemical Network Involved in Striatal Dopamine Signaling
Sammanfattning : In this thesis, I studied the molecular integration of reward-learning related neuromodulatory inputs by striatal medium-sized projection neurons (MSNs) using mass-action kinetic modeling.It is known that, in reward learning, an unexpected reward results in transient elevation in dopamine (peak) whereas omission of an expected reward leads to transient dopamine decrease (dip). LÄS MER
3. Hydrogels of conjugated polyelectrolytes for biosensor and biochip applications
Sammanfattning : This thesis describes the use of conjugated polyelectrolytes (CPEs) in biosensor devices. The method is based on non-covalent assembly of the biomolecule of interest and the CPE functioning as the reporter, in one case as a transducer, of biomolecular events. LÄS MER
4. Cell cycle regulation and DNA damage response : a record of polo-like kinase 1 activity
Sammanfattning : Division and cell proliferation is an essence of life. A human cell has in its core a very simple yet a very complex machinery to coordinate cell cycle activities and events. LÄS MER
5. Protein Interaction Studies with Low Molecular Weight Ligands : Applications for Drug Discovery, Basic Research and Diagnostic Tool Design
Sammanfattning : In this thesis, the interactions between different proteins and small ligands were characterized by surface plasmon resonance spectroscopy (SPR) and fluorescence resonance energy transfer (FRET) based assays. For the C-reactive protein (CRP), a new type of artificial binder was identified which allows designing diagnostic assays superior to commonly used standard assays. LÄS MER