Sökning: "Assay system"

Visar resultat 1 - 5 av 445 avhandlingar innehållade orden Assay system.

1. 1. The endocannabinoid system : a translational study from Achilles tendinosis to cyclooxygenase

   Författare :Emmelie Björklund; Christopher Fowler; Peter Zygmunt; Umeå universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES;

   Sammanfattning : The endogenous cannabinoids anandamide (arachidonoyl ethanolamide, AEA) and 2-arachidonoyl glycerol (2-AG) exert their effect by activating cannabinoid receptors (CB). These receptors mediate a broad range of physiological functions such as beneficial effects in pain and inflammation, although little is known about the expression of CB receptors in human pain conditions. LÄS MER

3. 2. Programmed Cell Death in Xylem Development

   Författare :Laetitia Charleen Courtois-Moreau; Hannele Tuominen; Stefan Jansson; Christina Dixelius; Umeå universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; PCD Programmed Cell Death ; Xylem; Apoptosis; Autophagy; Secondary Cell Walls; Microscopy; Microarrays; Comet Assay; TUNEL Assay; Cell and molecular biology; Cell- och molekylärbiologi;

   Sammanfattning : Concerns about climate changes and scarcity of fossil fuels are rising. Hence wood is becoming an attractive source of renewable energy and raw material and these new dimensions have prompted increasing interest in wood formation in trees, in both the scientific community and wider public. LÄS MER

4. 3. Improving cyanobacteria productivity: From theory to assay

   Författare :Kiyan Shabestary; Elton P. Hudson; Daniel Ducat; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; metabolic engineering; growth-coupling; growth-arrest; cyanobacteria; CRISPRi; CRISPRi library; flux balance analysis; genome-scale model; butanol; lactate; isoprene; Bioteknologi; Biotechnology;

   Sammanfattning : Bio-based production of biochemicals and biofuels holds great promises for the transition towards a more sustainable society. With increasing levels of carbon dioxide (CO2) in the atmosphere, cyanobacteria stand apart as natural catalysts directly converting CO2 and light to product. LÄS MER

5. 4. Monitoring of Cellular Dynamics with Focus on Electrochemical Detection Techniques

   Författare :Arto Heiskanen; Centrum för analys och syntes; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Microfluidic system; Microchip; PC12; Saccharomyces cerevisiae; Electrochemical impedance spectroscopy; Cell adhesion; Amperometry; Exocytosis; Redox environment; Cell-based assay;

   Sammanfattning : This thesis presents work in two main areas of focus, the development of electroanalytical techniques to monitor the dynamics of cellular processes as well as design and fabrication of microelectrode chips for electrochemical detection as well as a prototype microfluidic system to be used for cell analysis and culturing. The electrochemically monitored cellular parameters were the cellular redox environment (CRE), cell adhesion and exocytosis. LÄS MER

7. 5. Making Visible the Proximity Between Proteins

   Författare :Carl-Magnus Clausson; Ola Söderberg; Jens Coorssen; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Proximity ligation assay; In situ PLA; rolling circle amplification; protein interaction; protein-protein interaction; in situ; single cell; single molecule; protein complex; antibody; cancer; tissue section; microscopy; image analysis; system biology; multiplex; dynamic range; methods development; systems biology; Molekylär medicin; Molecular Medicine;

   Sammanfattning : Genomic DNA is the template of life - the entity which is characterized by a self-sustaining anatomical development, regulated signaling processes, the ability to reproduce and to respond to stimuli. Through what is classically known as the central dogma, the genome is transcribed into mRNA, which in turn is translated into proteins. LÄS MER