Sökning: "CRISPRi library"

Hittade 4 avhandlingar innehållade orden CRISPRi library.

  1. 1. Improving cyanobacteria productivity: From theory to assay

    Författare :Kiyan Shabestary; Elton P. Hudson; Daniel Ducat; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; metabolic engineering; growth-coupling; growth-arrest; cyanobacteria; CRISPRi; CRISPRi library; flux balance analysis; genome-scale model; butanol; lactate; isoprene; Bioteknologi; Biotechnology;

    Sammanfattning : Bio-based production of biochemicals and biofuels holds great promises for the transition towards a more sustainable society. With increasing levels of carbon dioxide (CO2) in the atmosphere, cyanobacteria stand apart as natural catalysts directly converting CO2 and light to product. LÄS MER

  3. 2. CRISPRi/a for investigating yeast tolerance to inhibitors in lignocellulosic hydrolysates

    Författare :Ibai Lenitz Etxaburu; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Lignocellulosic hydrolysates; ChIP-exo; Screening; Biosensor; Formic acid; Competitive growth assay; Yeast; Tolerance; CRISPRi a; Acetic acid;

    Sammanfattning : Saccharomyces cerevisiae has immense potential as a cell factory in various biotechnological processes where biomass from agricultural industry residues is used as feedstock. Nonetheless, the inhibitors released during the pretreatment of the biomass makes lignocellulosic hydrolysates a challenging substrate for microorganisms. LÄS MER

  4. 3. Exploring Saccharomyces cerevisiae’s responses to acetic acid and other inhibitors found in lignocellulosic hydrolysates

    Författare :Maurizio Mormino; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; acetic acid biosensor; formic acid; CRISPRi screening; acetic acid; Saccharomyces cerevisiae; lignocellulosic inhibitor tolerance; differential gene expression;

    Sammanfattning : The limited tolerance of the budding yeast Saccharomyces cerevisiae to lignocellulosic hydrolysate inhibitors is a key challenge to its use in biorefinery cell factories. Considerable resources have been invested in the isolation of yeast strains with better tolerance towards the inhibitors released during lignocellulose hydrolysis, such as acetic acid. LÄS MER

  5. 4. Droplet microfluidics for screening and sorting of microbial cell factories

    Författare :Sara Björk; Helene Svahn Andersson; Håkan Jönsson; Christoph Merten; KTH; []
    Nyckelord :Droplet microfluidics; Cell factories; High-throughput screening; Cell culture; Bioteknologi; Biotechnology;

    Sammanfattning : Cell factories are cells that have been engineered to produce a compound of interest, ranging from biopharmaceuticals to biofuels. With advances in metabolic engineering, the number of cell factory variants to evaluate has increased dramatically, necessitating screening methods with increased throughput. LÄS MER