Sökning: "verktyg"

Visar resultat 26 - 30 av 1000 avhandlingar innehållade ordet verktyg.

1. 26. Visualization of Protein Activity Status in situ Using Proximity Ligation Assays

   Författare :Malin Jarvius; Ulf Landegren; Ola Söderberg; Hans van Dam; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proximity ligation; in situ; high content screening; platelet-derived growth factor receptor; rolling circle amplification; protein interactions; in situ PLA; post-translational modifications; drug screening; Molecular biology; Molekylärbiologi; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Molekylär medicin; Molecular Medicine;

   Sammanfattning : In 2001 the human proteome organization (HUPO) was created with the ambition to identify and characterize all proteins encoded in the human genome according to several criteria; their expression levels in different tissues and under different conditions; the sub-cellular localization; post-translational modifications; interactions, and if possible also the relationship between their structure and function.When the knowledge of different proteins and their potential interactions increases, so does the need for methods able to unravel the nature of molecular processes in cells and organized tissues, and ultimately for clinical use in samples obtained from patients. LÄS MER

3. 27. Detection and Sequencing of Amplified Single Molecules

   Författare :Rongqin Ke; Mats Nilsson; Ulf Landegren; Radoje Drmanac; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; padlock probes; proximity ligation assay; rolling circle amplification; circle-to-circle amplification; single molecule detection; amplified single molecules; sequencing; in situ; single cell; Molecular Biotechnology; Molekylär bioteknik;

   Sammanfattning : Improved analytical methods provide new opportunities for both biological research and medical applications. This thesis describes several novel molecular techniques for nucleic acid and protein analysis based on detection or sequencing of amplified single molecules (ASMs). LÄS MER

4. 28. High Content Analysis of Proteins and Protein Interactions by Proximity Ligation

   Författare :Karl-Johan Leuchowius; Ola Söderberg; Ulf Landegren; Paul Goldsmith; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; in situ; proximity ligation; flow cytometry; high content screening; rolling circle amplification; in situ PLA; single-cell; single-molecule; protein interactions; drug screening; post-translational modifications; Molecular medicine; Molekylär medicin; Molekylär medicin; Molecular Medicine;

   Sammanfattning : Fundamental to all biological processes is the interplay between biomolecules such as proteins and nucleic acids. Studies of interactions should therefore be more informative than mere detection of expressed proteins. Preferably, such studies should be performed in material that is as biologically and clinically relevant as possible, i.e. LÄS MER

5. 29. Applications of in situ proximity ligation assays for cancer research and diagnostics

   Författare :Liza Löf; Masood Kamali-Moghaddam; Ulf Landegren; J.Paul Robinson; Uppsala universitet; []
   Nyckelord :Cancer; Diagnostics; Research; Prognostics; Method development; Flow cytometry; Drug delivery; Molekylär medicin; Molecular Medicine;

   Sammanfattning : In the field of cancer research and diagnostics it is crucial to have reliable methods for detecting molecules involved in the disease. New and better methods for diagnostics, prognostics and drug delivery therefore remain a permanent aim. LÄS MER

7. 30. Proximity Extension Assay : a universal tool for proteomics in precision medicine

   Författare :Phathutshedzo M. Muthelo; Ulf Landegren; Jochen Schwenk; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Proximity Extension Assay; Precision medicine; Assay development; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi;

   Sammanfattning : Precision medicine aims to utilise biomarkers to ensure the right treatment is given to the right patient at the right time. In pursuit of this goal, sensitive protein detection is essential to improve opportunities for discovery, verification, validation and development of valuable protein biomarkers. LÄS MER