Sökning: "total internal reflection fluorescence TIRF microscopy"

Visar resultat 6 - 9 av 9 avhandlingar innehållade orden total internal reflection fluorescence TIRF microscopy.

1. 6. Assay development for studies of G protein-coupled receptors at the single-molecule level

   Författare :Olov Wahlsten; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TIRF microscopy; colocalization; drug discovery; QCM-D; GPCR; pharmacodynamics; kinetics; equilibrium fluctuation analysis;

   Sammanfattning : G protein-coupled receptors (GPCRs), also known as seven transmembrane (7TM) receptors,is the largest family of cell surface receptors. These receptors play a key role in transmittinga wide variety of signals across the cell membrane and are involved in physiologicalprocesses such as sensory transduction, cell-cell communication, neuronal transmission,and hormonal signaling. LÄS MER

3. 7. Virus-cell membrane interactions - Binding studies of Herpes Simplex Virus using surface-sensitive techniques

   Författare :Nadia Peerboom; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TIRF microscopy; single particle tracking; binding kinetics; glycosaminoglycans; herpes simplex virus;

   Sammanfattning : Viruses are parasites capable of infecting all forms of life. They lack the ability to replicate by themselves and therefore hijack the replication machinery of cells to produce new viral copies, called virions. LÄS MER

4. 8. Tethered Lipid Vesicles as Nanoscale Sensor Elements

   Författare :Seyed Tabaei; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; aquaglyceroporin; lipid vesicles; total internal reflection fluorescence TIRF microscopy; cell membrane; aquaporin; pore formation; surface plasmon resonance SPR ; phospholipase A2; antiviral peptide; lipid bilayer; biosensing; microfluidics;

   Sammanfattning : The primary aim of this thesis work has been to explore and advance the potential of surface tethered lipid vesicles as a platform for studying in particular the following three biomembrane-related processes: trans-membrane transport, membrane-peptide interactions and the activity of membrane active enzymes. First, a method based on surface plasmon resonance (SPR) spectroscopy was developed for studying passive diffusion processes as well as the membrane-protein mediated transport of small non electrolyte molecules across lipid bilayers. LÄS MER

5. 9. Mechanisms of Integrin Signal Transduction

   Författare :Anne Stefansson; Staffan Johansson; Annika Armulik; Björn Öbrink; Uppsala universitet; []
   Nyckelord :Cell biology; Integrin; Integrin signalling; PI3K; Akt PKB; Integrin transmembrane domain; cell spreading; Cellbiologi;

   Sammanfattning : Integrins are a protein family of cell surface receptors, expressed in all cell types in the human body, except the red blood cells. Besides their importance in mediating physical connections with the surrounding environment, the integrin family members are also vital signalling mediators. LÄS MER