Sökning: "reaction specificity"

Visar resultat 11 - 15 av 130 avhandlingar innehållade orden reaction specificity.

1. 11. Quantum Chemical Studies of Chemotherapeutic Drug Cisplatin : Activation and Binding to DNA

   Författare :Johan Raber; David van der Spoel; Paolo Carloni; Uppsala universitet; []
   Nyckelord :Quantum chemistry; cisplatin; quantum chemistry; DNA; density functional theory; activation; JM118; substitution reaction; Platinum; guanine; adenine; cytostatic drug; aquation; anation; activation; Kvantkemi;

   Sammanfattning : The serendipitous discovery of the potent cytotoxic properties of cisplatin brought about a revolution in the treatment of certain types of cancer, but almost fifty years later, there still remain unknown areas in the chemistry of cisplatin. There are questions regarding which form of the drug reaches its DNA target, or why certain DNA sequences are more preferred than others for reaction with cisplatin. LÄS MER

3. 12. Allosteric Regulation and Radical Transfer in Ribonucleotide Reductase

   Författare :Karl-Magnus Larsson; Pär Nordlund; Derek Logan; Roger Kornberg; Stockholms universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; allosteric regulation; substrate specificity; ribonucleotide reductase; radical; Biochemistry; Biokemi;

   Sammanfattning : The stability of biological life over time requires that the integrity of the genetic material of an organism, the genome, be maintained as it is passed on from one generation to the next. All known cellular life has DNA-based genomes, which are duplicated before each cell division in a process called replication. LÄS MER

4. 13. The structural basis for the catalytic specificity of manganese lipoxygenases : 3D structure analysis of the lipoxygenase of Magnaporthe oryzae

   Författare :Anneli Wennman; Ernst H. Oliw; Marcia Newcomer; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; oxylipin; lipoxygenase; crystal structure; crystallography; HPLC; mass spectrometry; yeast; site-directed mutagenesis; fungi; Biochemical Pharmacology; Biokemisk farmakologi;

   Sammanfattning : Lipoxygenases (LOX) catalyze regio- and stereospecific oxygenation of polyunsaturated fatty acids to hydroperoxides. These hydroperoxides are further metabolized to leukotrienes and lipoxins in mammals, and are involved in asthma and inflammation. LOX of animals and plants contain iron as catalytic metal (FeLOX). LÄS MER

5. 14. Building blocks for polymer synthesis by enzymatic catalysis

   Författare :Stefan Semlitsch; Mats Martinelle; Sergio Riva; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Enzyme; Enzyme Engineering; Biocatalysis; Lipase; CalB; MsAcT; Substrate specificity; Selectivity; Polymer Chemistry; Polymer Synthesis; Bioteknologi; Biotechnology;

   Sammanfattning : The search for alternatives to oil-based monomers has sparked interest for scientists to focus on the use of renewable resources for energy production, for the synthesis of polymeric materials and in other areas. With the use of renewable resources, scientists face new challenges to first isolate interesting molecules and then to process them. LÄS MER

7. 15. Redesign of substrate specificity of glutathione transferase and glutathione reductase : Enzyme engineering by directed mutagenesis, phage-display selection and DNA shuffling

   Författare :Lars O. Hansson; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

   Sammanfattning : Human glutathione transferase (GST) A1-1 was expressed in fusion with the phage gene IIIproduct and a library of phage-displayed GST mutants was constructed by randomization of fouractive-site residues. Variant GSTs were isolated by mechanism-based phage display selection, using an immobilized transition-state analog of a nucleophilic aromatic substitution (SNAr) reaction. LÄS MER