Sökning: "reaction specificity"

Visar resultat 6 - 10 av 130 avhandlingar innehållade orden reaction specificity.

  1. 6. DNA-Assisted Immunoassays for High-Performance Protein Analyses

    Författare :Junhong Yan; Masood Kamali-Moghaddam; Ulf Landegren; Christer Wingren; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proximity ligation assay; magnetic beads; conjugation; phosphorylation; affinity binder; protein complexes; recombinant binder; sensitivity; specificity; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

    Sammanfattning : Proteins play important roles in most cellular functions, such as, replication, transcription regulation, signal transduction, for catalyzing chemical reaction, etc. Technologies developed to identify proteins rely either on observing their own properties such as charge, size, mass to charge ratio or sequence composition; or on using affinity reagents that recognize specific protein targets. LÄS MER

  3. 7. Acute pancreatitis. With special reference to the activation peptide from carboxypeptidase B (CAPAP) as a predictor of severity

    Författare :Stefan Appelros; Kirurgi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; accuracy; exocrine dysfunction; radioimmunoassay; CAPAP; carboxypeptidase B; activation peptide; aetiology; prediction of severity; morbidity; mortality; severe acute pancreatitis; Incidence; acute pancreatitis; sensitivity; specificity; Surgery; orthopaedics; traumatology; Kirurgi; ortopedi; traumatologi;

    Sammanfattning : There is a wide range in the reported annual incidence of acute pancreatitis (AP). A minority of these patients develop a severe disease characterised by a high mortality and morbidity. In order to improve the outcome for patients with an attack of severe AP it is important to identify these patients early in the course of disease. LÄS MER

  4. 8. Nucleotide Binding to Trimeric dUTPase: Studies on Recombinant Enzymes using Kinetic and Spectroscopic Methods

    Författare :Johan Nord; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; C-terminus; dUTPase; EIAV; Escherichia coli; flexibility; Homo sapiens; kinetics; NMR; nucleotide metabolism; rate constants of lig-and binding; production of recombinant enzyme; dUTP; specificity; Biochemistry; Metabolism; Biokemi;

    Sammanfattning : Recombinant dUTPase from the bacterium Escherichia coli, the retrovirus equine infectious anemia virus (EIAV) and Homo sapiens have been made available for physico-chemical characterisation. The protocols developed have also proven to be suitable for production of enzymes modified by isotope labelling or other methods. LÄS MER

  5. 9. Cutting Edge – Cleavage Specificity and Biochemical Characterization of Mast Cell Serine Proteases

    Författare :Ulrika Karlson; Lars Hellman; Urban Gullberg; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biology; mast cell; serine protease; tryptase; chymase; substrate specificity; phage display; Biologi; Biology; Biologi; Molecular Immunology; molekylär immunologi;

    Sammanfattning : It is well established that mast cells (MC) are key players in airway pathologies such as allergic asthma, but they are also known to contribute to host defense and tissue remodeling. MC serine proteases are the major protein components of mast cell granules and accordingly, are most likely involved in many aspects of MC function. LÄS MER

  7. 10. Rational engineering of esterases for improved amidase specificity in amide synthesis and hydrolysis

    Författare :Peter Hendil-Forssell; Mats Martinelle; Romas J. Kazlauskas; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Amidase; Biocatalysis; Enzyme; Esterase; Enzyme engineering; Lipase; Substrate specificity; Bioteknologi; Biotechnology;

    Sammanfattning : Biocatalysis is an ever evolving field that uses enzymes or microorganisms for chemical synthesis. By utilizing enzymes that generally have evolved for specific reactions under mild conditions and temperatures, biocatalysis can be a more environmentally friendly option compared to traditional chemistry. LÄS MER