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Visar resultat 1 - 5 av 8 avhandlingar som matchar ovanstående sökkriterier.

1. 1. Systems Biology of Protein Secretion in Human Cells: Multi-omics Analysis and Modeling of the Protein Secretion Process in Human Cells and its Application

   Författare :Rasool Saghaleyni; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Erythropoietin; cancer protein secretory pathway; protein secretion modeling; HEK293; protein secretion; Integrative omics analysis; genome-scale modeling;

   Sammanfattning : Since the emergence of modern biotechnology, the production of recombinant pharmaceutical proteins has been an expanding field with high demand from industry. Pharmaceutical proteins have constituted the majority of top-selling drugs in the pharma industry during recent years. LÄS MER

3. 2. Systems biology of protein synthesis and secretion in yeast

   Författare :Qi Qi; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; recombinant protein production; protein synthesis; multi-omics analysis; protein secretion; genome-scale modeling;

   Sammanfattning : Protein synthesis and secretion is a vital process to maintain cell function. As it demands numerous building blocks, cofactors and chaperones generated from metabolism and translation, the process is intertwined with metabolic and regulatory networks. LÄS MER

4. 3. Constraint-based modeling of yeast metabolism and protein secretion

   Författare :Feiran Li; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; protein secretion; metabolism; yeast evolution; genome-scale metabolic model; phenotype diversity;

   Sammanfattning : Yeasts are extensively exploited as cell factories for producing alcoholic beverages, biofuels, bio-pharmaceutical proteins, and other value-added chemicals. To improve the performance of yeast cell factories, it is necessary to understand their metabolism. LÄS MER

5. 4. Engineering yeast for improved recombinant protein production

   Författare :Yanyan Wang; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; point mutation; genome-scale modeling; recombinant protein; omics analysis; CRISPR Cas9; protein secretion; Saccharomyces cerevisiae;

   Sammanfattning : Recombinant proteins are broadly used from basic research to therapeutic development and include industrial enzymes and pharmaceutical proteins. The increasing demand for improved production and enhanced quality of recombinant proteins requires robust biotech-based strategies to overcome the limitations of protein extraction from natural sources. LÄS MER

7. 5. Physiology of Saccharomyces cerevisiae Producing Recombinant Insulin in Continuous Culture

   Författare :Ali Kazemi Seresht; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; unfolded protein response; Keywords: heterologous protein production; prolonged chemostat; transcriptome; insulin analogue precursor; metabolic adaptation; URA3d; plasmid copy number; promoter truncation; high cell density cultivation; TPI1;

   Sammanfattning : The yeast Saccharomyces cerevisiae has widely been used as a host for the production of heterologous proteins. High-level production of heterologous proteins is likely to impose a metabolic burden on the host cell and can thus affect various aspects of cellular physiology. LÄS MER