Sökning: "targeted proteomics"

Visar resultat 1 - 5 av 65 avhandlingar innehållade orden targeted proteomics.

  1. 1. Neuropeptidomics – Expanding Proteomics Downwards

    Författare :Marcus Svensson; Per Andrén; Per Svenningsson; Jonathan Sweedler; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pharmaceutical pharmacology; proteomics; peptidomics; mass spectrometry; neuropeptide; chromatography; Farmaceutisk farmakologi; PHARMACY; FARMACI;

    Sammanfattning : Biological function is mainly carried out by a dynamic population of proteins which may be used as markers for disease diagnosis, prognosis, and as a guide for effective treatment. In analogy to genomics, the study of proteins is called proteomics and it is generally performed by two-dimensional gel electrophoresis and mass spectrometric methods. LÄS MER

  3. 2. Multiplexed absolute quantification of blood plasma proteins using targeted proteomics

    Författare :David Kotol; Mathias Uhlén; Edfors Fredrik; Roman Zubarev; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; mass spectrometry; targeted proteomics; stable isotope standards; recombinant protein standards; absolute quantification; blood plasma; multiplex analysis; plasma profiling; Biotechnology; Bioteknologi;

    Sammanfattning : Proteins are the molecular building blocks of all living organisms. They are the functioning actors in metabolism and communication, and they give specific architecture and purpose to all tissues. Their abundance is dynamic and can be interpreted and associated to different physiological states or diseases. LÄS MER

  4. 3. Targeted multiplex proteomics for risk stratification in patients with cardiovascular disease

    Författare :Emma Skau; Pär Hedberg; Jerzy Leppert; Johan Ärnlöv; Eva Swahn; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; cardiovascular disease; proteomics; myocardial infarction; peripheral arterial disease; biomarker; GDF-15; Cardiology; Kardiologi;

    Sammanfattning : Risk stratification is valuable in patients with cardiovascular disease (CVD). Proteins involved in different pathophysiological processes in atherosclerosis have shown prognostic capacity. A new technology, Proximity Extension Assay (PEA), enables the simultaneous analysis of numerous plasma proteins from a minimal amount of plasma. LÄS MER

  5. 4. Targeted proteomics methods for protein quantification of human cells, tissues and blood

    Författare :Fredrik Edfors; Mathias Uhlén; Albert Heck; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; proteomics; mass spectrometry; protein quantification; stable isotope standard; parallel reaction monitoring; immuno-enrichment; Bioteknologi; Biotechnology;

    Sammanfattning : The common concept in this thesis was to adapt and develop quantitative mass spectrometric assays focusing on reagents originating from the Human Protein Atlas project to quantify proteins in human cell lines, tissues and blood. The work is based around stable isotope labeled protein fragment standards that each represent a small part of a human protein-coding gene. LÄS MER

  7. 5. Membrane Protein Biogenesis in Escherichia coli : A proteomics approach

    Författare :David Vikström; Jan-Willem de Gier; Eitan Bibi; Stockholms universitet; []
    Nyckelord :SRP; YidC; SecE; Membrane protein biogenesis; protein targeting; 2D BN SDS-PAGE; Biochemistry; biokemi;

    Sammanfattning : In the bacterium Escherichia coli all proteins are synthesized in the cytoplasm. However, at least 25% of them then need to be exported to another cellular compartment where they exert their function. E. coli has several different targeting pathways to ensure the correct localization of its proteins. LÄS MER