Sökning: "protein purification"
Visar resultat 16 - 20 av 265 avhandlingar innehållade orden protein purification.
16. Expression and purification of membrane proteins: Focus on the G-protein coupled receptor MC4r
Sammanfattning : Membrane proteins are crucial components of the cell and are involved in many biological processes. Recombinant overexpression systems together with different purification methods are necessary to obtain large amounts of purified receptor for biophysical and functional studies. LÄS MER
17. Purification, characterization and immunological studies of rat urinary proteins causing allergy in humans
Sammanfattning : Rats are among the most frequently used laboratory animals and allergy to them constitutes a common occupational problem. Approximately 20-30% of the persons engaged in work with laboratory animals acquire symptoms of allergy. LÄS MER
18. Exploration of analytical methods to study motif-mediated host-virus protein-protein interactions
Sammanfattning : Proteins are responsible for countless processes in living creatures, but most often they do not perform these tasks alone. Rather, they engage in interactions with other proteins, creating whole protein-protein interaction (PPI) networks. LÄS MER
19. Biosensing and Protein Array Design using Lipid Assemblies
Sammanfattning : Biomolecular sensing often includes immobilization of biomolecules, e.g. DNA, antibodies etc., to solid supports, where they act as selective recognition elements. LÄS MER
20. Protein production, characterization and structure determination in structural genomics
Sammanfattning : This thesis covers the process from expression of a heterologous gene in Escherichia coli to structure determination of a protein by nuclear magnetic resonance (NMR) spectroscopy. The first part concerns structural genomics-related parallel screening studies on the effect of fusion tags (in particular the His tag) on protein solubility and the use of fusion tags in fast, parallel purification protocols intended for initial biophysical characterization of human proteins produced in E. LÄS MER