Sökning: "protein analyses"

Visar resultat 1 - 5 av 626 avhandlingar innehållade orden protein analyses.

  1. 1. Structure determination and thermodynamic stabilization of an engineered protein-protein complex

    Författare :Elisabet Wahlberg; Per-Åke Nygren; Bengt-Harald Jonsson; KTH; []
    Nyckelord :ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; protein structure; coupled folding; NMR spectroscopy; protein stability; protein-protein interactions; binding thermodynamics; isothermal titration calorimetry; protein engineering; Structural biochemistry; Strukturbiokemi;

    Sammanfattning : The interaction between two 6 kDa proteins has been investigated. The studied complex of micromolar affinity (Kd) consists of the Z domain derived from staphylococcal protein A and the related protein ZSPA-1, belonging to a group of binding proteins denoted affibody molecules generated via combinatorial engineering of the Z domain. LÄS MER

  2. 2. Proximity Ligation Assay for High Performance Protein Analysis in Medicine

    Författare :Gucci Jijuan Gu; Ulf Landegren; Thomas Joos; Uppsala universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; protein; post-translational modification; interactions; protein domain; western blotting; MARK; tau; AD; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

    Sammanfattning : High quality reagents are preconditions for high performance protein analyses. But despite progress in some techniques, e.g. mass spectrometry, there is still a lack of affinity-based detection techniques with enhanced precision, specificity, and sensitivity. LÄS MER

  3. 3. Protein Misfolding in Human Diseases

    Författare :Karin Almstedt; Per Hammarström; Daniel Otzen; Linköpings universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Misfolding; carbonic anhydrase; prion protein; protein stability; Biochemistry; Biokemi;

    Sammanfattning : There are several diseases well known that are due to aberrant protein folding. These types of diseases can be divided into three main categories:Loss-of-function diseasesGain-of-toxic-function diseasesInfectious misfolding diseases Most loss-of-function diseases are caused by aberrant folding of important proteins. LÄS MER

  4. 4. Angiopoietin-like protein 4 : an unfolding chaperone regulating lipoprotein lipase activity

    Författare :Valentina Sukonina; Gunilla Olivecrona; Lena Kjellen; Umeå universitet; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Lipoprotein lipase; angiopoietin-like protein 4; protein folding; human adipose tissue; macrophages; peroxisome proliferator-activated receptor delta PPARδ ; Medical microbiology; Medicinsk mikrobiologi;

    Sammanfattning : Lipoprotein lipase (LPL) is the main enzyme hydrolyzing triglyceride-rich lipoproteins in plasma. Proteoglycan-bound LPL on the vascular endothelium represent the functional pool of active enzyme. LPL is regulated in a tissue specific manner according to metabolic demands. LÄS MER

  5. 5. Immobilized biomembrane chromatographic analyses : with reference to membrane protein purification and reconstitution

    Författare :Lars Haneskog; Uppsala universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP;

    Sammanfattning : .... LÄS MER