Sökning: "nucleic acid delivery"

Visar resultat 6 - 10 av 35 avhandlingar innehållade orden nucleic acid delivery.

  1. 6. Cell-penetrating peptides and bioactive cargoes : Strategies and mechanisms

    Författare :Kalle Kilk; Ülo Langel; Michael J. Gait; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Cell-penetrating peptide; delivery; Peptide nucleic acid; Biochemistry; Biokemi;

    Sammanfattning : The cell membrane is an impermeable barrier for most macromolecules. Recently discovered cell-penetrating peptides (CPPs) have gained lot of attention because they can cross the membrane, and even more, carry cargoes with them. How CPPs enter cells is still not clear, while the delivery of different cargoes has been convincingly shown. LÄS MER

  3. 7. Condensation of DNA for Gene Delivery: Studies on Vector-Nucleic Acid Interactions and Transfection Efficiency

    Författare :Kristina Fant; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; dendrimer; endocytosis; uptake; gene delivery; optical spectroscopy; structure-activity relationship; DNA; cell-penetrating peptide;

    Sammanfattning : This thesis concerns the delivery of nucleic acids into cells and tissues with the aim to change or correct gene expression. The field of gene delivery has the ultimate goal of producing genetic drugs for clinical applications, and the activity in this area is intense although so far the use is limited to research purposes. LÄS MER

  4. 8. Unravelling the structure and dynamics of lipid nanoparticles for biomolecule delivery

    Författare :Jennifer Gilbert; NanoLund: Centre for Nanoscience; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; lipid nanoparticles; neutron scattering; SAXS; protein encapsulation; nucleic acid delivery; lipid self-assembly;

    Sammanfattning : Encapsulation in self-assembled lipid structures has been shown to increase the stability of large biomolecules, including proteins and nucleic acids (NAs), which has many potential applications in the food and pharmaceutical industries. In order to fully optimise the encapsulation system for a certain application, however, it is important to understand the effect of the encapsulated biomolecule on the lipid structure and vice versa. LÄS MER

  5. 9. Nucleic acid delivery : reports from the search of the magic bullet

    Författare :Joana Viola; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Gene therapy is regarded as one of the most promising therapeutic approaches, since it has the potential to treat disorders by correcting malformations at the nucleic acids level rather than proteins, as opposed to conventional medicine. However, for non-viral gene therapy to successfully fulfill the requirements associated to the “magic bullet” has proven difficult. LÄS MER

  7. 10. Chimeric gene delivery vectors : Design, synthesis, and mechanisms from transcriptomics analysis

    Författare :Moataz Dowaidar; Ülo Langel; Sandrine Sagan; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Cell-penetrating peptides; magnetic nanoparticles; graphene oxide; autophagy; siRNA; SCO; QSAR; neurokemi med molekylär neurobiologi; Neurochemistry with Molecular Neurobiology;

    Sammanfattning : Delivery of nucleic acid is a promising approach for genetic diseases/disorders. However, gene therapy using oligonucleotides (ONs) suffers from low transfection efficacy due to negative charges, weak cellular permeability, and enzymatic degradation. LÄS MER