Sökning: "mutational analysis"

Visar resultat 16 - 20 av 116 avhandlingar innehållade orden mutational analysis.

  1. 16. Evolutionary Dynamics of Mutation and Gene Transfer in Bacteria

    Författare :Peter A Lind; Dan I Andersson; J. Arjan G. M. de Visser; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; fitness cost; bacterial evolution; gene amplification; mutational biases; GC content; synonymous substitutions; horizontal gene transfer; experimental evolution; Bacteriology; Bakteriologi; Microbiology; Mikrobiologi; Genetics; Genetik; Biology; Biologi; Mikrobiologi; Microbiology;

    Sammanfattning : The study of bacterial evolution is fundamental for addressing current problems of antibiotic resistance and emerging infectious diseases and lays a solid foundation for successful and rational design in biotechnology and synthetic biology. The main aim of this thesis is to test evolutionary hypotheses, largely based on theoretical considerations and sequence analysis, by designing scenarios in a laboratory setting to obtain experimental data. LÄS MER

  3. 17. Facilitating precision medicine through analysis of next-generation sequencing projects

    Författare :Qingyang Xiao; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Precision medicine constitutes an emerging strategy that aims at the individualization of healthcare by considering the personal molecular features and environmental factors of the patient in question. Genetic biomarkers constitute one dimension of a patient’s molecular phenotype that can allow for treatment stratification. LÄS MER

  4. 18. Computational Prediction and Analysis of Protein-Carbohydrate and Protein-Protein Interactions

    Författare :Ashok Krishna Chaitanya Koppisetty; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Arylsulfatase-A; Norovirus VA387; Molecular Dynamics; Saposin B; Computational Docking;

    Sammanfattning : The structure and energetics of protein-carbohydrate and protein-protein interactions are of great interest due to their importance in many biological phenomena. Two cases of protein-carbohydrate interactions and one case of protein-protein interactions were predicted and analyzed using existing computational methods. LÄS MER

  5. 19. HPRT mutational spectra and microsatellite DNA instability in HNPCC and lung cancer patients

    Författare :Peter Hackman; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Somatic gene mutation; mutational spectra; HPRT gene; lung cancer; smoking; hMLH1 gene; HNPCC; microsatellite instability; mismatch repair; T-cells;

    Sammanfattning : The, general aims of this work have been to explore the use of microsatellite DNA length variation and mutational spectra of the hypoxanthine guanine phosphoribosyltransferase (HPRT) locus in T-cells as tools for a better understanding of human somatic mutagenesis in vivo. In particular (a) how inherited mismatch repair (MMR) deficiency may affect the stability of microsatellite DNA and the mutational spectrum at the HPRT locus, and (b) how the mutational spectrum at the HPRT locus is influenced by smoking, and by predisposition to lung cancer among never smokers. LÄS MER

  7. 20. Detection and analysis of genetic alterations in normal skin and skin tumours

    Författare :Åsa Sivertsson; KTH; []
    Nyckelord :single cell; DNA sequencing; p53; mutation; UV; BCC; pyrosequencing; malignant melanoma; N-ras;

    Sammanfattning : The investigation of genetic alterations in cancer-relatedgenes is useful for research, prognostic and therapeuticpurposes. However, the genetic heterogeneity that often occursduring tumour progression can make correct analysischallenging. LÄS MER