Sökning: "Vincent Bulone"

Visar resultat 6 - 10 av 12 avhandlingar innehållade orden Vincent Bulone.

1. 6. Identification and characterisation of chitin and cellulose synthases in oomycetes : New tools for biochemical studies and structure determination

   Författare :Stefan Klinter; Vincent Bulone; Lauren S. McKee; Mirjam Czjzek; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; biochemical characterisation; cellulose synthase; chitin synthase; in vitro activity assay; microtubule-interacting and trafficking domain; nanoparticle; oomycetes; phylogenetic analysis; yeast engineering; biokemisk karakterisering; cellulosasyntas; kitinsyntas; in vitro aktivitetsanalys; mikrotubulus-interagerande och -trafikerande domän; nanopartikel; oomyceter; fylogenetisk analys; jäst proteinteknik; Bioteknologi; Biotechnology;

   Sammanfattning : Despite resembling ‘true’ fungi in terms of morphological features, oo­mycetes form a distinct eukaryotic lineage of filamentous microorganisms that belongs to the stramenopiles, a group of protists also comprising the closely-related brown algae and diatoms. Many oomycetes are devastating pathogens of plants and animals, globally causing significant economic los­ses in the agriculture and aquaculture industries, and posing considerable environmental damage to natural ecosystems. LÄS MER

3. 7. Strategies for the Discovery of Carbohydrate-Active Enzymes from Environmental Bacteria

   Författare :Johan Larsbrink; Vincent Bulone; Harry Brumer; Birte Svensson; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; CAZyme discovery; xyloglucan; polysaccharide-utilisation locus; microbiota; α-xylosidase; GH31; transglucosidase; human gut; SRA - Molekylär biovetenskap; SRA - Molecular Bioscience;

   Sammanfattning : The focus of this thesis is a comparative study of approaches in discovery of carbohydrate-active enzymes (CAZymes). CAZymes synthesise, bind to, and degrade all the multitude of carbohydrates found in nature. LÄS MER

4. 8. Understanding and manipulating primary cell walls in plant cell suspension cultures

   Författare :Felicia Leijon; Vincent Bulone; Katja Salomon Johansen; KTH; []
   Nyckelord :Callose synthase; carbohydrate-binding module; cell wall engineering; cellulose microfibril; cellulose nanocrystal; glycosyltransferase; mass spectrometry; plasmodesmata; Populus; primary cell wall; radiometric assay; spectrophotometric assay; Biotechnology; Bioteknologi;

   Sammanfattning : The cell wall is required for many aspects of plant function and development. It is also an accessible and renewable resource utilized both in unrefined forms and as raw material for further development. Increased knowledge regarding cell wall structure and components will contribute to better utilization of plants and the resources they provide. LÄS MER

5. 9. Analyzing the properties and biosynthesis of β-glucans from Gluconacetobacter and poplar

   Författare :Erik Malm; Vincent Bulone; Paul Dupree; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Cellulose synthesis; Bioinformatics; Proteomics; Cell wall;

   Sammanfattning : Glucans are polysaccharides integral to many materials and biological functions. Under the umbrella of Biomime, the Swedish Center for Biomimetic Fiber Engineering, this work has aimed to improve basic understanding of the biosynthesis of such glucans. LÄS MER

7. 10. Probing Interactions between Bio-Fibre Components

   Författare :Niklas Nordgren; Mark Rutland; Harry Brumer; Vincent Bulone; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; AFM; QCM-D; cellulose; xyloglucan; surface forces; friction; colloidal probe; nanotribology; biomimetic; enzymatic; polysaccharide; self assembly; grafting; thiol; spring constant; calibration; photodetector calibration.; Surface and colloid chemistry; Yt- och kolloidkemi;

   Sammanfattning : I den här avhandlingen har atomkrafts mikroskopi (AFM) och kvartskristall mikrovåg med dissipation (QCM-D) används för att undersöka interaktioner i bio-fiber modell system av cellulosa och xyloglukan (XG); en naturligt förekommande polysackarid viktig för tillväxt av träd och andra växter. Dessutom har det avgörande momentet av friktionskrafts-kalibrering studerats för att erhålla noggranna och kvantitativa resultat med kolloidal prob AFM. LÄS MER