Sökning: "Ultracentrifugation"

Visar resultat 21 - 25 av 27 avhandlingar innehållade ordet Ultracentrifugation.

1. 21. Purification and biodistribution of extracellular vesicles

   Författare :Joel Z Nordin; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : Extracellular vesicles (EVs) are nano-sized vesicles that contain bioactive lipids, RNAs and proteins, which can be transferred to recipient cells. EVs are important for physiological as well as pathological processes, such as coagulation and immune homeostasis, aiding cancer metastasis and spread of infectious diseases. LÄS MER

3. 22. Protein mass spectrometry in the drug discovery process

   Författare :Agneta Tjernberg; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :Mass spectrometry; ESI-MS; MALDI-TOF MS; drug discovery; protein characterization; dissociation constants;

   Sammanfattning : Most targets for drug discovery are proteins. Drug discovery includes target identification and validation, structural biology, lead generation, lead optimisation, pre-clinical development and clinical trials. The overall aim of this thesis is to apply protein mass spectrometry (MS) in the early drug discovery process. LÄS MER

4. 23. Postprandial lipaemia and its relation to premature atherosclerosis in middle-aged men

   Författare :Susanna Boquist; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :alimentary lipaemia; atherosclerosis; atorvastatin; coronary heart disease; intima-media thickness; triglyceride-rich lipoproteins;

   Sammanfattning : The present research programme was set up to investigate whether pertubations of the metabolism of postprandial triglyceride-rich lipoproteins (TRLs) are related to premature atherosclerosis and to determine if treatment with a 3-hydroxy-3-methylglutaryl-coenzyme A reductase inhibitor (atorvastatin) improves deranged alimentary lipaemia in postinfarction patients with combined hyperlipidaemia. The relation between plasma insulin and alimentary lipaemia was investigated as well. LÄS MER

5. 24. Functional and structural properties of eukaryotic DNA polymerase epsilon

   Författare :Olga Chilkova; Erik Johansson; Per Elias; Umeå universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; eukaryotic DNA replication; DNA polymerase epsilon; protein-DNA interaction; Biochemistry; Biokemi;

   Sammanfattning : In eukaryotes there are three DNA polymerases which are essential for the replication of chromosomal DNA: DNA polymerase alpha (Pol alpha), DNA polymerase delta (Pol delta) and DNA polymerase epsilon (Pol epsilon). In vitro studies of viral DNA replication showed that Pol alpha and Pol delta are sufficient for DNA replication on both leading and lagging DNA strands, thus leaving the function of Pol epsilon unknown. LÄS MER

7. 25. Developmental and stress induced changes in peptide and catecholamine content of the paraaortic paraganglia

   Författare :Lars-Magnus Wikström; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :paraganglia; adrenal gland; enkephalin; galanin; NPY; catecholamines; development; asphyxia; hypoglycemia; reserpine;

   Sammanfattning : The paraaortic body (PAB) is the largest extra-adrenal paraganglion and during the perinatal period it contains a substantial amount of catecholamines (CAs). The PAB also contain peptides, whose function in the PAB is not clear. LÄS MER