Sökning: "protein-DNA interaction"

Visar resultat 1 - 5 av 14 avhandlingar innehållade orden protein-DNA interaction.

  1. 1. Modelling the Protein-DNA Interface

    Författare :Malin Lüking; Shina C. Lynn Kamerlin; Ehmke Pohl; Uppsala universitet; []
    Nyckelord :Protein-DNA interactions; Molecular Dynamics; Coarse-grained simulations; gene regulation; Biokemi; Biochemistry;

    Sammanfattning : Protein-DNA interactions are crucial to life. Several millions of DNA base pair steps are organ- ised, read and protected by proteins in every cell. Protein-DNA interactions must be specific, controllable and reasonably fast. Understanding how these features coexist is one of the great challenges for biochemists and molecular biologists. LÄS MER

  3. 2. Thermodynamics of protein-DNA interactions

    Författare :Thomas Lundbäck; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :DNA-protein interactions; molecular recognition; thermodynamics; specificity.dehydration; salt dependence.;

    Sammanfattning : This thesis treats the molecular recognition between DNA-binding proteins and DNA and the way these interactions are affected by the surrounding water solution and dissolved solutes. The work involves studies on the thermodynamics of sequence specific DNA-binding of the human glucocorticoid receptor DNA-binding domain and the nonsequence specific DNA- binding of Sso7d from the archaeon Sulfolobus solfataricus. LÄS MER

  4. 3. Reaction-Diffusion kinetics of Protein DNA Interactions

    Författare :Anel Mahmutovic; Johan Elf; David van der Spoel; Pieter Rein ten Wolde; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; umbrella sampling; molecular dynamics; RDME; PDE; sliding; intersegment transfer; hopping; sterics; intersegment transfer; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

    Sammanfattning : Transcription factors need to rapidly find one specific binding site among millions of nonspecific sites on the chromosomal DNA. In this thesis I use various aspects of reaction-diffusion theory to investigate the interaction between proteins and DNA and to explain the searching, finding and binding to specific operator sites. LÄS MER

  5. 4. Visualizing Interacting Biomolecules In Situ

    Författare :Irene Weibrecht; Ola Söderberg; Ulf Landegren; Banafshe Larijani; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proximity ligation; in situ PLA; padlock probe; rolling circle amplification; flow cytometry; in situ; single cell; single molecule; protein interaction; protein-DNA interaction; posttranslational modification; Molecular medicine; Molekylär medicin; Molekylär medicin; Molecular Medicine;

    Sammanfattning : Intra- and intercellular information is communicated by posttranslational modifications (PTMs) and protein-protein interactions, transducing information over cell membranes and to the nucleus. A cells capability to respond to stimuli by several highly complex and dynamic signaling networks provides the basis for rapid responses and is fundamental for the cellular collaborations required in a multicellular organism. LÄS MER

  7. 5. Functional and structural properties of eukaryotic DNA polymerase epsilon

    Författare :Olga Chilkova; Erik Johansson; Per Elias; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; eukaryotic DNA replication; DNA polymerase epsilon; protein-DNA interaction; Biochemistry; Biokemi;

    Sammanfattning : In eukaryotes there are three DNA polymerases which are essential for the replication of chromosomal DNA: DNA polymerase alpha (Pol alpha), DNA polymerase delta (Pol delta) and DNA polymerase epsilon (Pol epsilon). In vitro studies of viral DNA replication showed that Pol alpha and Pol delta are sufficient for DNA replication on both leading and lagging DNA strands, thus leaving the function of Pol epsilon unknown. LÄS MER