Sökning: "Protein production"

Visar resultat 6 - 10 av 1301 avhandlingar innehållade orden Protein production.

1. 6. Membrane and secretory protein production in Escherichia coli

   Författare :Alexandros Karyolaimos; Jan-Willem de Gier; Matthew DeLisa; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; recombinant protein; tunable protein production; cytoplasmic membrane; periplasm; signal peptide; force profile analysis; co-translational folding; Biochemistry; biokemi;

   Sammanfattning : Escherichia coli (E. coli) is the most widely used bacterium for the production of recombinant proteins. However, the production of proteins in its cytoplasmic membrane or periplasm is challenging. Therefore, the aim of this doctoral thesis was to develop setups for enhancing the production of membrane and secretory proteins in E. LÄS MER

3. 7. Engineering microbial cell factories for protein production

   Författare :Kiavash Mirzadeh; Daniel Daley; Grzegorz Kudla; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Protein production; expression vector; recombinant DNA; Translation initiation region; Escherichia coli; mRNA secondary structure; Synthetic evolution; Biochemistry; biokemi;

   Sammanfattning : Proteins are often produced using microbial cell factories for academic or industrial purposes. Protein production is however not an open-and-shut procedure. Production yields often vary in an unpredictable and context dependent manner, limiting the rational design of a straightforward production experiment. LÄS MER

4. 8. Engineering yeast for improved recombinant protein production

   Författare :Yanyan Wang; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; point mutation; genome-scale modeling; recombinant protein; omics analysis; CRISPR Cas9; protein secretion; Saccharomyces cerevisiae;

   Sammanfattning : Recombinant proteins are broadly used from basic research to therapeutic development and include industrial enzymes and pharmaceutical proteins. The increasing demand for improved production and enhanced quality of recombinant proteins requires robust biotech-based strategies to overcome the limitations of protein extraction from natural sources. LÄS MER

5. 9. Protein production in the E. coli cell envelope

   Författare :Thomas Baumgarten; Jan-Willem de Gier; Dirk-Jan Scheffers; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; E. coli; protein biogenesis; recombinant proteins; membrane proteins; secretory proteins; protein displays; outer membrane vesicles; Biochemistry; biokemi;

   Sammanfattning : Proteins fulfil essential functions in every cell and malfunctioning proteins are often the cause of diseases. On the other hand, proteins like antibody fragments or hormones can be used to treat diseases. LÄS MER

7. 10. Systems biology of protein synthesis and secretion in yeast

   Författare :Qi Qi; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; recombinant protein production; protein synthesis; multi-omics analysis; protein secretion; genome-scale modeling;

   Sammanfattning : Protein synthesis and secretion is a vital process to maintain cell function. As it demands numerous building blocks, cofactors and chaperones generated from metabolism and translation, the process is intertwined with metabolic and regulatory networks. LÄS MER