Sökning: "Joakim Lundeberg"

Visar resultat 16 - 20 av 41 avhandlingar innehållade orden Joakim Lundeberg.

  1. 16. Library Preparation for High Throughput DNA Sequencing

    Författare :Anders Jemt; Joakim Lundeberg; Petri Auvinen; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA; RNA; sequencing; massively parallel sequencing; library preparation; automation; genome; transcriptome; Bioteknologi; Biotechnology;

    Sammanfattning : Order 3 billion base pairs of DNA in the correct order and you get the blueprint of a human, the genome. Before the introduction of massively parallel sequencing a little more than a decade ago it would cost around $10 million to get this blueprint. LÄS MER

  3. 17. On Transcriptome Sequencing

    Författare :Daniel Klevebring; Joakim Lundeberg; Ulf Gyllensten; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Transcriptome; RNA-seq; DNA sequencing; gene expression profiling; non-coding RNA; small RNA; Functional genomics; Funktionsgenomik; Bioinformatics; Bioinformatik; Genetics; Genetik; Molecular biology; Molekylärbiologi; Cell biology; Cellbiologi;

    Sammanfattning : This thesis is about the use of massive DNA sequencing to investigate the transcriptome. During recent decades, several studies have made it clear that the transcriptome comprises a more complex set of biochemical machinery than was previously believed. LÄS MER

  4. 18. The Spatial Context – through the lens of method development

    Författare :Linda Kvastad; Joakim Lundeberg; Lars Feuk; KTH; []
    Nyckelord :RNA; RNA-sequencing; transcriptomics; spatial transcriptomics; single cells; pediatric brain tumors; Spatial RIN; Bioteknologi; Biotechnology;

    Sammanfattning : In the present moment of time, we find ourselves in a period where the advancement of genomic tools is progressing at a fast pace. Of particular interest for this thesis is the study of gene activity. LÄS MER

  5. 19. Arrayed identification of DNA signatures

    Författare :Max Käller; Lundeberg Joakim; Mats Nilsson; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; apyrase; allele-specific extension; competitive hybridization; DNA sequencing; genotyping; human papillomavirus HPV ; MC1R; microarray; mutation; p53; protease; Bioengineering; Bioteknik;

    Sammanfattning : In this thesis techniques are presented that aim to determine individual DNA signatures by controlled synthesis of nucleic acid multimers. Allele-specific extension reactions with an improved specificity were applied for several genomic purposes. Since DNA polymerases extend some mismatched 3’-end primers, an improved specificity is a concern. LÄS MER

  7. 20. Mapping Transcriptomes in Tissues

    Författare :Ludvig Larsson; Joakim Lundeberg; Stefania Giacomello; Naveed Ishaque; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; spatially resolved transcriptomics; spatial transcriptomics; transcriptomics; data analysis; rumsligt upplöst transkriptomik; rumslig transkriptomik; transkriptomik; dataanalys; Biotechnology; Bioteknologi;

    Sammanfattning : Over the past few decades, the advent of pioneering biotechnological methods has allowed scientists to analyze the molecular components of multicellular organisms with remarkable precision. The field of transcriptomics has witnessed a rapid development of technologies for gene expression profiling of biological samples. LÄS MER