Sökning: "Immunoblotting methods"

Visar resultat 1 - 5 av 17 avhandlingar innehållade orden Immunoblotting methods.

  1. 1. Helicobacter pylori cell surface interactions with glycosaminoglycans. Identification and characterisation of proteins binding to heparin/heparan sulphate

    Författare :Meeme Utt; Celiaki och diabetes; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; SELDI-TOF MS; bioinformatics; proteomics; immunoblotting; 2-DE; biosensor; heparan sulphate; heparin; binding; Helicobacter pylori; cell surface; bakteriologi; Mikrobiologi; mycology; bacteriology; Microbiology; mykologi; virologi; virology;

    Sammanfattning : Helicobacter pylori is a gastric pathogen which cause chronic type B gastritis and peptic ulcer disease. H. pylori produces virulence factors such as urease, vacuolating cytotoxin VacA, cag pathogenicity island-associated proteins, flagella and adhesins. LÄS MER

  3. 2. Studies of immune responses to cell surface proteins of Helicobacter pylori and Borrelia burgdorferi by enzyme immunoassay and immunoblotting

    Författare :Ingrid Nilsson; Avdelningen för medicinsk mikrobiologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Mikrobiologi; mycology; virology; B. burgdorferi; H.pylori; cell surface proteins; gel-electrophoresis; enzyme immunoassay; Western immunoblotting; bacteriology; Microbiology; bakteriologi; virologi; mykologi;

    Sammanfattning : Helicobacter pylori and Borrelia burgdorferi s.l. can cause chronic infections by evading the immune system. These two human pathogens express a number of immunogenic cell surface proteins. LÄS MER

  4. 3. The Impact of Drugs of Abuse on the Neuroproteome : Application of Immunoblotting and LC-MS-based Proteomics

    Författare :Uwe Rossbach; Pierre Le Grevès; Fred Nyberg; Matthias Hallberg; Jan Kehr; Uppsala universitet; []
    Nyckelord :;

    Sammanfattning : Qualitative and quantitative analysis of proteins can be based either on immunological or on mass spectrometry-utilizing methods. In this thesis, different approaches of protein analysis were applied to elucidate the impact of drugs of abuse on the rat brain. Androgens have been reported to affect mood, anxiety and memory function. LÄS MER

  5. 4. Development of proteomic methods for studying cerebrospinal fluid proteins involved in Alzheimer´s disease

    Författare :Maja Puchades; Göteborgs universitet; []
    Nyckelord :Alzheimer s disease; cerebrospinal fluid; proteomics; mass spectrometry; biomarkers; synaptic proteins; liquid phase IEF; biomarkers;

    Sammanfattning : Alzheimer's disease (AD) is the most common cause of dementia in western countries. The main neuropathological findings in the AD brain are senile plaques, neurofibrillary tangles and degeneration of neurons and synapses. LÄS MER

  7. 5. Helicobacter pylori bacterial diversity and human disease : microbiological and epidemiological studies with special reference to gastric cancer

    Författare :Helena Enroth; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Helicobacter Pylori; microbiology; epidemiology; diversity; gastric adenocarcinoma; risk factors; coccoid; genomic fingerprinting; strain variation; multiple strain infection; PCR; 2-D PAGE; immunoblotting; CagA; VacA;

    Sammanfattning : The aims of this thesis were to explore if the coccoid form of H. pylori is a degenerative, dead form of the bacteria. We also wanted to study if multiple strain infections of H. pylori existed in Swedish subjects, if the strain variations were connected to certain disease groups, and if specific protein markers could be found in H. LÄS MER