Sökning: "Fluorescence microscopy"

Visar resultat 11 - 15 av 312 avhandlingar innehållade orden Fluorescence microscopy.

1. 11. Laser Scanning Multiphoton Microscopy – Focusing on Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Imaging for Biomedical Applications

   Författare :Jeemol James; Göteborgs universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Laser scanning multiphoton microscopy; intrinsic cellular fluorophores.; fluorescence correlation spectroscopy; fluorescence lifetime imaging; melanoma metastasis;

   Sammanfattning : Laser scanning multiphoton microscopy (MPM) is considered as a non-invasive technology for three-dimensional imaging of complex biological tissues. The quantitative potential of the MPM is not investigated as much as qualitative imaging. LÄS MER

3. 12. Automating STED microscopy for functional and structural live-cell imaging

   Författare :Jonatan Alvelid; Ilaria Testa; Eggeling Christian; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; STED; microscopy; nanoscopy; super-resolution microscopy; automation; image analysis; fluorophores; Biologisk fysik; Biological Physics;

   Sammanfattning : Optical microscopy imaging methods are today invaluable tools for studies in life sciences as they allow visualization of biological systems, tissues, cells, and sub-cellular compartments from millimetres down to nanometres. The invention and development of nanoscopy in the past 20 years has pushed fluorescence microscopy down to the nanoscale, reaching beyond the natural diffraction limit of light that does not allow focusing of visible light below sizes of around 200 nm, and into the realm of what was previously only thought possible with electron microscopy. LÄS MER

4. 13. Multiphoton Microscopy Targeting Tissue Development. Towards the real-time imaging of epidermal differentiation

   Författare :Monika Malak; Göteborgs universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; multiphoton microscopy; fluorescence lifetime imaging; epidermal differentiation; reconstructed human epidermis; organ-on-a-chip; intrinsic fluorescence;

   Sammanfattning : The development of novel organ-on-a-chip models calls for methodologies, which will allow the study of tissue development and pharmacological outcome in real-time. Multiphoton microscopy (MPM) has been proposed as a suitable technique for noninvasive imaging of live specimens, utilizing intrinsic tissue fluorescence. LÄS MER

5. 14. Development of Techniques for Characterization, Detection and Protein Profiling of Extracellular Vesicles

   Författare :Sara Cavallaro; Jan Linnros; Apurba Dev; Hakho Lee; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; extracellular vesicles; streaming current; fluorescence microscopy; atomic force microscopy; scanning electron microscopy; protein profiling; size profiling; Biological and Biomedical Physics; Biologisk och biomedicinsk fysik;

   Sammanfattning : Nanosized extracellular vesicles (EVs, ∼30-2000 nm) have emerged as important mediators of intercellular communication, offering opportunities for both diagnostics and therapeutics. In particular, small EVs generated from the endolysosomal pathway (∼30-150 nm), referred to as exosomes, have attracted interest as a suitable biomarker for cancer diagnostics and treatment monitoring based on minimally invasive liquid biopsies. LÄS MER

7. 15. Fluorescence Studies of Cell Division in Escherichia coli

   Författare :Bill Söderström; Gunnar von Heijne; Jie Xiao; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; E. coli; Cell division; FtsZ; Fluorescence microscopy; FRAP; Biophysics; biofysik;

   Sammanfattning : In Escherichia coli the cell division is carried out by a large dynamic protein complex called the divisome. The divisome assembles in a two-step manner starting with the localization of the eukaryotic tubulin homologue FtsZ to the midcell. Together with other early arriving proteins FtsZ form an intermediate structure called the Z-ring. LÄS MER