Sökning: "Analytical model"

Visar resultat 21 - 25 av 1173 avhandlingar innehållade orden Analytical model.

  1. 21. Improved Techniques for Protein Analysis Focusing on Membrane Proteins and Hydrophobic Peptides

    Författare :Theres Redeby; Åsa Emmer; Jonas ergquist; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Integral membrane proteins; Hydrophobic peptides; Sample handling; MALDI-TOF-MS; Structured sample support; CE; fraction collection; microchannel; Analytical chemistry; Analytisk kemi;

    Sammanfattning : In this thesis, the vital cell functions performed by integral membrane proteins (IMPs) are briefly discussed. Such proteins are under-represented in most protein studies due to the hydrophobic nature of IMPs, which seriously complicate their solubilization, sample handling, preparation, separation and analysis. LÄS MER

  3. 22. New Concepts for Dielectrophoretic Separations and Dielectric Measurements of Bioparticles

    Författare :Fredrik Aldaeus; Johan Roeraade; Peter Stilbs; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; Analytisk kemi;

    Sammanfattning : This thesis presents two new concepts for separation of micro particles using dielectrophoresis, demonstrated by calculated examples, as well as a new method for obtaining dielectric data on living cells. The thesis is based on four papers. LÄS MER

  4. 23. Enzyme Immunoassay in Combination with Liquid Chromatography for Sensitive and Selective Determination of Drugs in Biosamples

    Författare :Ulf Lövgren; Centrum för analys och syntes; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; amplification; alkaline phosphatase; enzyme immunoassay; liquid chromatography; flow-injection; micellar; Analytisk kemi;

    Sammanfattning : Two different sensitive microtitre-plate based enzyme immunoassays were developed and evaluated for determination of the model drug compound, budesonide, in blood plasma. In the first approach, high sensitivity was obtained by using amperometric detection in a flow injection system for determination of the enzyme product p-aminophenol. LÄS MER

  5. 24. Affinity biosensors based on a capacitive transducer

    Författare :Christine Berggren; Centrum för analys och syntes; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; self-assembled monolayers; capacitance; heavy metal ion; non-labeled; nucleic acid; DNA biosensor; Biosensor; immunosensor; Analytical chemistry; Analytisk kemi;

    Sammanfattning : Capacitive biosensors were developed for several analytes such as hormones, interleukines, viruses and heavy metal ions. The sensing surfaces were prepared by immobilizing the biorecognition element to self-assembled monolayers (SAMs) on gold. SAMs form spontaneously on gold by adsorption of thiols, sulfides or disulfides. LÄS MER

  7. 25. Improved techniques for CE-MALDI-MS off-line coupling and MALDI-MS analysis of primarily hydrophobic proteins and peptides

    Författare :Johan Jacksén; Åsa Emmer; Anna Karin/Minna Borg Karlsson/Hakkarainen; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Capillary electrophoresis; Hydrophobic peptides; Matrix-Assisted Laser Desorption Ionization Mass Spectrometry; Prestructured target; Off-line interface; Silicon microcanal; Matrix; Protein cleavage digestion.; Kapillärelektrofores; Hydrofoba peptider; Matris-assisterad laserdesorptions-joniserings-masspektrometri; Strukturbelagd provplatta; Off-line interface; Mikrokanal i kisel; Matris; Proteinklyvning -spjälkning.; Analytical chemistry; Analytisk kemi;

    Sammanfattning : Due to the hydrophobic nature of integral membrane proteins (IMP) they give rise to several difficulties concerning handling and analysis, which is not the case for the most water soluble proteins. New analysis methods are needed, where the insolubility problems of the hydrophobic proteins due to aggregation and adhesion are tackled. LÄS MER