Sökning: "50S subunit"
Visar resultat 1 - 5 av 11 avhandlingar innehållade orden 50S subunit.
1. Structural and Genetic Studies of Translation in Escherichia coli
Sammanfattning : Ribosomes are the universal ribonucleoprotein organelles that translate the genetic message from mRNA to protein. In prokaryotes, the ribosomal subunits are 30S and 50S subunit, which bind together during the translation process forming 70S ribosome. LÄS MER
2. The importance of maturation factors in 30S ribosomal subunit assembly
Sammanfattning : The assembly of the ribosome is a complex process that needs to be highly efficient to support maximum growth. Although the individual subunits of the ribosome can be reconstituted in vitro, such a reaction is inefficient in comparison to the assembly rate in vivo. LÄS MER
3. Studies on the functional interaction of translation initiation factor IF1 with ribosomal RNA
Sammanfattning : Translation initiation factor IF1 is a small, essential and ubiquitous protein factor encoded by a single infA gene in bacteria. Although several important functions have been attributed to IF1, the precise reason for its indispensability is yet to be defined. LÄS MER
4. Ribosomes and subunits from Escherichia coli studied by asymmetrical flow field-flow fractionation
Sammanfattning : In this thesis asymmetrical flow field-flow fractionation (FFF) separations of ribosomes and subunits have been studied. Ribosomal material was prepared from Escherichia coli (E. coli) cells and analyzed by the size separation technique. LÄS MER
5. Finding the unknowns in trans-translation
Sammanfattning : Ribosomes stalled on problematic mRNAs can be rescued by a mechanism called trans-translation. This mechanism employs a dual transfer-messenger RNA molecule (tmRNA) together with a helper protein (SmpB). In this work we have used an in vitro translation system with pure components to further clarify the roles of tmRNA and SmpB in trans-translation. LÄS MER