Sökning: "recombinant protein standards"

Visar resultat 1 - 5 av 7 avhandlingar innehållade orden recombinant protein standards.

  1. 1. High-throughput protein analysis using mass spectrometry-based methods

    Författare :Tove Boström; Sophia Hober; Thomas Laurell; Ole N. Jensen; KTH; []
    Nyckelord :Proteomics; mass spectrometry; affinity proteomics; immunoenrichment; immunoprecipitation; IMAC; screening; protein production; protein purification; ISET; quantification; SILAC; stable isotope standard; antibody validation; Bioteknologi; Biotechnology;

    Sammanfattning : In the field of proteomics, proteins are analyzed and quantified in high numbers. Protein analysis is of great importance and can for example generate information regarding protein function and involvement in disease. Different strategies for protein analysis and quan- tification have emerged, suitable for different applications. LÄS MER

  3. 2. Multiplexed absolute quantification of blood plasma proteins using targeted proteomics

    Författare :David Kotol; Mathias Uhlén; Edfors Fredrik; Roman Zubarev; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; mass spectrometry; targeted proteomics; stable isotope standards; recombinant protein standards; absolute quantification; blood plasma; multiplex analysis; plasma profiling; Biotechnology; Bioteknologi;

    Sammanfattning : Proteins are the molecular building blocks of all living organisms. They are the functioning actors in metabolism and communication, and they give specific architecture and purpose to all tissues. Their abundance is dynamic and can be interpreted and associated to different physiological states or diseases. LÄS MER

  4. 3. Targeted proteomics methods for protein quantification of human cells, tissues and blood

    Författare :Fredrik Edfors; Mathias Uhlén; Albert Heck; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; proteomics; mass spectrometry; protein quantification; stable isotope standard; parallel reaction monitoring; immuno-enrichment; Bioteknologi; Biotechnology;

    Sammanfattning : The common concept in this thesis was to adapt and develop quantitative mass spectrometric assays focusing on reagents originating from the Human Protein Atlas project to quantify proteins in human cell lines, tissues and blood. The work is based around stable isotope labeled protein fragment standards that each represent a small part of a human protein-coding gene. LÄS MER

  5. 4. Process Analytical Tools for the Monitoring and Characterization of Bacterial Toxins

    Författare :Martin Hedström; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Bioteknik; Biotechnology; protein degradation; mass spectrometry; Bacterial toxins; Biosensor;

    Sammanfattning : In the era of proteomics, glycomics and ultimately metabolomics, with constantly increasing knowledge regarding the human biosystem, the number of specialized therapeutic agents which will be developed against different diseases is predicted to be on the upswing. An increasing portion of these new drugs will, according to recent predictions (Labrou, 2003), be of proteinaceous origin produced according to biotechnological standards. LÄS MER

  7. 5. Leukotriene A4 hydrolase : identification of amino acid residues involved in catalyses and substrate-mediated inactivation

    Författare :Martin J Mueller; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Leukotriene (LT) A4 hydrolase catalyzes the committed step in the biosynthesis of LTB4, a classical chemoattractant and immuine-modulating lipid mediator involved in inflammation, host-defense against infections, and systemic, PAF-mediated, lethal shock. LTA4 hydrolase is a bifunctional zinc metalloenzyme with a chloride stimulated arginyl aminopeptidase activity. LÄS MER