Sökning: "protein quantification"
Visar resultat 1 - 5 av 210 avhandlingar innehållade orden protein quantification.
1. High-throughput protein analysis using mass spectrometry-based methods
Sammanfattning : In the field of proteomics, proteins are analyzed and quantified in high numbers. Protein analysis is of great importance and can for example generate information regarding protein function and involvement in disease. Different strategies for protein analysis and quan- tification have emerged, suitable for different applications. LÄS MER
2. Metal Affinity Peptides in Biotechnological Applications As tools in protein purification, protein stabilisation, metal quantification and metal tolerance of bacteria and plants
Sammanfattning : Metal affinity tags have been fused to proteins and demonstrated to function as tools in protein purification, protein stabilisation, quantitative metal analysis and metal tolerance of bacteria and plants. In biotechnological applications proteins frequently have to function in nonnatural environments with harsh conditions which impose requirements for high stability on the proteins. LÄS MER
3. Targeted proteomics methods for protein quantification of human cells, tissues and blood
Sammanfattning : The common concept in this thesis was to adapt and develop quantitative mass spectrometric assays focusing on reagents originating from the Human Protein Atlas project to quantify proteins in human cell lines, tissues and blood. The work is based around stable isotope labeled protein fragment standards that each represent a small part of a human protein-coding gene. LÄS MER
4. On the Development of Mucin-based Biomaterial Coatings
Sammanfattning : Owing to their key role in mucosal functioning as surface barriers with biospecific interaction potentials, the mucins are interesting candidates for use as surface modifiers in biomaterials applications. In this work, “mild” fractionation procedures were used to prepare mucins of bovine (BSM), porcine (PGM), and human (MG1) origin. LÄS MER
5. Towards specific quantification of exosomes using surface-based sensing
Sammanfattning : When exosomes were first observed in 1967, they were believed to be cell debris until extensive studies demonstrated key biological functionalities related to their composition and formation mechanism. Exosomes are nanometer-sized lipid vesicles of endocytic origin, with a membrane consisting of lipids and proteins and encapsulating a cargo composed of soluble proteins and genetic material. LÄS MER