Sökning: "parallel reaction monitoring"

Visar resultat 1 - 5 av 15 avhandlingar innehållade orden parallel reaction monitoring.

1. 1. Targeted proteomics methods for protein quantification of human cells, tissues and blood

   Författare :Fredrik Edfors; Mathias Uhlén; Albert Heck; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; proteomics; mass spectrometry; protein quantification; stable isotope standard; parallel reaction monitoring; immuno-enrichment; Bioteknologi; Biotechnology;

   Sammanfattning : The common concept in this thesis was to adapt and develop quantitative mass spectrometric assays focusing on reagents originating from the Human Protein Atlas project to quantify proteins in human cell lines, tissues and blood. The work is based around stable isotope labeled protein fragment standards that each represent a small part of a human protein-coding gene. LÄS MER

3. 2. Advances in DNA Detection

   Författare :Yajing Song; Olof Emanuelsson; Jonas Tegenfeldt; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; DNA detection; active filter paper; visual detection; throughput; fluorescence; superparamagnetic beads; rare DNA; Q-PCR; chemical fragmentation; DNA library preparation; massively parallel sequencing; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Sammanfattning : DNA detection technologies have an increasing importance in our everyday lives, with applications ranging from microbial diagnostics to forensic analysis, food safety evaluation, and environmental monitoring. Currently, as the associated costs decrease, DNA diagnostic techniques are routinely used in research laboratories, in clinical and forensic practice. LÄS MER

4. 3. Proteomic strategies for blood biomarker development in rare dystrophinopathies

   Författare :Camilla Johansson; Cristina Al-Khalili Szigyarto; Ola Söderberg; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Duchenne muscular dystrophy; Becker muscular dystrophy; proteomics; affinity-proteomics; biomarkers; DIA-MS; SRM-MS; immunoassay; suspension bead arrays; Duchenne muskeldystrofi; Becker muskeldystrofi; proteomik; affinitetsproteomik; biomarkörer; DIA-MS; SRM-MS; immunoassay; suspension bead array; Biotechnology; Bioteknologi;

   Sammanfattning : Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD) are two rare genetic disorders of the family dystrophinopathy. They are both caused by the lack of, or reduced production of, the protein dystrophin. LÄS MER

5. 4. Mass Spectrometry and Affinity Based Methods for Analysis of Proteins and Proteomes

   Författare :Mårten Sundberg; Margareta Ramström; Jonas Bergquist; Christer Wingren; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Mass spectrometry; proteomics; microarray; protein; antibody; antigen; affinity; validation; Kemi med inriktning mot analytisk kemi; Chemistry with specialization in Analytical Chemistry;

   Sammanfattning : Proteomics is a fast growing field and there has been a tremendous increase of knowledge the last two decades. Mass spectrometry is the most used method for analysis of complex protein samples. It can be used both in large scale discovery studies as well as in targeted quantitative studies. LÄS MER

7. 5. On the continuous inter-group interaction speed improvement in large-scale agile software development for embedded software

   Författare :Antonio Martini; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

   Sammanfattning : An open issue in industry is how to scale-up Agile Software Development in large companies developing embedded systems. Intra-team interaction is well supported by established Agile practices, while how to handle the feedback for the Agile team from other teams and other parts of the organization is still unexplored. LÄS MER