Sökning: "protein labeling"

Visar resultat 1 - 5 av 118 avhandlingar innehållade orden protein labeling.

  1. 1. The Interaction Between the Chaperonin GroEL and Human Carbonic Anhydrase II : Protein Folding and Stability Studies

    Författare :Malin Persson; Linköpings universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; carbonic anhydrase; protein folding; protein stability; chaperone; GroEL; folding mechanism; site-directed spinlabeling; electron paramagnetic resonance EPR ;

    Sammanfattning : The presence of GroEL/ES during the refolding of human carbonic anhydrase II was found to increase the yield of active enzyme from approx. 70 to approx. 100%. This chaperone action on the enzyme could be obtained by adding GroEL alone, and the time course in that case was only moderately slower than the spontaneous process. LÄS MER

  2. 2. Fluorescent Probes and Protein Misfolding: Methods and Applications

    Författare :Irem Nasir; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Protein misfolding; fluorophores; filter-trap; site-specific fluorophore labeling; amyloid-beta; microscopic rate constants; solvatochromic probes; alpha-synuclein; lipid vesicles; nanoparticles; protein adsorption;

    Sammanfattning : Protein misfolding is a frequently occurring event in living cells and without the quality control, the consequences are disasterous. In this thesis, we studied protein misfolding in vitro by employing a variety of fluorophores, to either detect the highly ordered aggregates of intrinsically disordered proteins/peptides or slight misfolding of a natively folded protein. LÄS MER

  3. 3. Protein Microarray Chips

    Författare :Goran Klenkar; Bo Liedberg; Brian MacCraith; Linköpings universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Microarray; biosensor; biomolecular interaction; narcotics detection; imaging ellipsometry and surface plasmon resonance; Molecular biophysics; Molekylär biofysik;

    Sammanfattning : Livet tas för givet av de flesta. Det finns däremot många som ägnar stora delar av sitt liv för att försöka lösa dess mysterier. En del av lösningen ligger i att förstå hur alla molekyler är sammanlänkade i det gigantiska nätverk som definierar den levande organismen. LÄS MER

  4. 4. Molecular characterization of protein phosphorylation in plant photosynthetic membranes

    Författare :Maria Hansson; Alexander V. Vener; Jean-David Rochaix; Linköpings universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; protein phosphorylation; photosynthesis; mass spectrometry; protein characterization; Cell biology; Cellbiologi;

    Sammanfattning : Higher plants cannot move to a more favorable place when the environmental conditions are changing. To adapt to changes in light, temperature and access to water the plants had to evolve special mechanisms at the molecular level. LÄS MER

  5. 5. Isotope labeling of proteins for spectroscopic studies

    Författare :Emil Gustavsson; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Isotope labeling; Proteins; Cell-free protein expression; NMR; IR; Infrared;

    Sammanfattning : Protein structural dynamics have been closely tied to the molecular mechanisms controlling for example enzyme activities. In my work, I have mainly studied the bacteriophytochrome from Deinococcus radiodurans. LÄS MER