Sökning: "GroEL"

Visar resultat 1 - 5 av 13 avhandlingar innehållade ordet GroEL.

1. 1. The Interaction Between the Chaperonin GroEL and Human Carbonic Anhydrase II : Protein Folding and Stability Studies

   Författare :Malin Persson; Linköpings universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; carbonic anhydrase; protein folding; protein stability; chaperone; GroEL; folding mechanism; site-directed spinlabeling; electron paramagnetic resonance EPR ;

   Sammanfattning : The presence of GroEL/ES during the refolding of human carbonic anhydrase II was found to increase the yield of active enzyme from approx. 70 to approx. 100%. This chaperone action on the enzyme could be obtained by adding GroEL alone, and the time course in that case was only moderately slower than the spontaneous process. LÄS MER

3. 2. Structural rearrangements of actins interacting with the Chaperonin systems TRiC/Prefoldin and GroEL/ES

   Författare :Laila Villebeck; Bengt-Harald Jonsson; Bente Vestergaard; Linköpings universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; chaperonin mechanisms; bound target protein; fluorescence resonance energy transfer FRET ; Actin; abundant eukaryotic protein; Molecular biology; Molekylärbiologi;

   Sammanfattning : The studies in this thesis are mainly focused on the effects that the chaperonin mechanisms have on a bound target protein. Earlier studies have shown that the bacterial chaperonin GroEL plays an active role in unfolding a target protein during the initial binding. LÄS MER

4. 3. Studies on endoribonuclease RNase E and its role in RNA turnover

   Författare :Björn Sohlberg; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :Gene regulation RNA processing mRNA stability endoribonucleases RNase E AUUUA-motifs Archaea;

   Sammanfattning : RNA turnover is an important level of regulation of gene expression, by which a cell can rapidly adjust to changes in its environment. The turnover of RNA is done by an efficient but not well understood mechanism. LÄS MER

5. 4. Biophysical Studies of Protein Folding, Aggregation and Interactions with Molecular Chaperones

   Författare :Per Hammarström; Linköpings universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

   Sammanfattning : The information for a protein to fold correctly into a well defined three-dimensional functional structure is embedded in the primary sequence. Much effort has been devoted to characterize the nature of the unfolded state, since this state represents the starting point of the folding reaction. LÄS MER

7. 5. Biophysical studies of protein folding upon interaction with molecular chaperones

   Författare :Satish Babu Moparthi; Uno Carlsson; Per Hammarström; Bengt-Harald Jonsson; Pernilla Wittung‐Stafshede; Linköpings universitet; []
   Nyckelord :NATURAL SCIENCES; NATURVETENSKAP;

   Sammanfattning : Proteins are biological macromolecules that serve all functions in cells. Every protein consists of a sequence of amino acids that is folded into a three‐dimensional structure to maintain the unique information it contains and to allow the protein to perform its specific actions. LÄS MER