Sökning: "hammerhead"

Hittade 4 avhandlingar innehållade ordet hammerhead.

  1. 1. Expression and function of ribozymes in transgenic mice

    Författare :Michael Andäng; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :hammerhead ribozyme; hairpin ribozyme; transgenic mice; HIV-1; pseudotype virus; cleavage products; cis-cleavage;

    Sammanfattning : Hammerhead and hairpin ribozymes are short RNAs that act as sequence-specific endoribonucleases by cleaving a target RNA after hybridization to complementary sequences. By changing the specific target hybridization sequence of the ribozyme, virtually any RNA can be targeted, making ribozymes a versatile tool for gene down-regulation (knockdown). LÄS MER

  3. 2. Preclinical studies of ribozyme-mediated gene therapy for HIV-1

    Författare :Catharina Maijgren Steffensson; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :AIDS; antiretroviral; cis-cleavage; gene therapy; HAART; hairpin; hammerhead; HIV-1; in vitro; in vivo; ribozyme; RNA;

    Sammanfattning : Approximately 40 million people are infected with HIV-1 and AIDS is the number one cause of death in Africa as well as, the fourth leading cause of death globally. Patients in developed countries generally receive highly active antiretroviral therapy (HAART), even though the drugs are expensive and sometimes cause side-effects and treatment failure. LÄS MER

  4. 3. Some Aspects of Nucleic Acids Chemistry

    Författare :Edouard Zamaratski; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Bioorganic chemistry; Bioorganisk kemi; Bioorganic chemistry; Bioorganisk kemi; bioorganisk kemi; Biorganic Chemistry;

    Sammanfattning : This thesis is divided into two parts based on a total of 8 papers: Part 1: Synthesis, physicochemical and biochemical studies of chemically modified oligonucleotides and their duplexes and triplexes. Potency of the chromophore conjugated DNA oligonucleotides as antigene and antisense gene repressors was evaluated. LÄS MER

  5. 4. Translational accuracy, ribozyme efficiency and NAD metabolism in Escherichia coli

    Författare :Martin Stancek; Leif Isaksson; Lars Hederstedt; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; translational accuracy; nicotinate mononucleotide adenylyltransferase; Microbiology; Mikrobiologi;

    Sammanfattning : Interactions between the translational machinery and mRNA in Escherichia coli have been studied. In the first section nonsense codon readthrough and changed translational reading frame were measured in different growth phases. LÄS MER