Sökning: "hammerhead ribozyme"

Hittade 3 avhandlingar innehållade orden hammerhead ribozyme.

1. 1. Preclinical studies of ribozyme-mediated gene therapy for HIV-1

   Författare :Catharina Maijgren Steffensson; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :AIDS; antiretroviral; cis-cleavage; gene therapy; HAART; hairpin; hammerhead; HIV-1; in vitro; in vivo; ribozyme; RNA;

   Sammanfattning : Approximately 40 million people are infected with HIV-1 and AIDS is the number one cause of death in Africa as well as, the fourth leading cause of death globally. Patients in developed countries generally receive highly active antiretroviral therapy (HAART), even though the drugs are expensive and sometimes cause side-effects and treatment failure. LÄS MER

3. 2. Translational accuracy, ribozyme efficiency and NAD metabolism in Escherichia coli

   Författare :Martin Stancek; Leif Isaksson; Lars Hederstedt; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; translational accuracy; nicotinate mononucleotide adenylyltransferase; Microbiology; Mikrobiologi;

   Sammanfattning : Interactions between the translational machinery and mRNA in Escherichia coli have been studied. In the first section nonsense codon readthrough and changed translational reading frame were measured in different growth phases. LÄS MER

4. 3. Expression and function of ribozymes in transgenic mice

   Författare :Michael Andäng; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :hammerhead ribozyme; hairpin ribozyme; transgenic mice; HIV-1; pseudotype virus; cleavage products; cis-cleavage;

   Sammanfattning : Hammerhead and hairpin ribozymes are short RNAs that act as sequence-specific endoribonucleases by cleaving a target RNA after hybridization to complementary sequences. By changing the specific target hybridization sequence of the ribozyme, virtually any RNA can be targeted, making ribozymes a versatile tool for gene down-regulation (knockdown). LÄS MER