Sökning: "fed-batch technique"
Visar resultat 1 - 5 av 11 avhandlingar innehållade orden fed-batch technique.
1. The temperature-limited fed-batch technique for control of Escherichia coli cultures
Sammanfattning : The objective of this study was to investigate the physiology and productivity in Escherichia colicultures with emphasis on the temperature-limited fed-batch (TLFB) culture. The TLFB techniquecontrols the oxygen consumption rate of the growing culture by a gradually declining temperaturefrom 37-35 °C down to 20-18 °C. LÄS MER
2. Probing Control : Analysis and Design with Application to Fed-Batch Bioreactors
Sammanfattning : In most control problems the objective is to control the output at a desired value in spite of disturbances. In some cases, the best setpoint is not known a priori and it should be found online to optimize the process performance. This thesis examines a probing strategy that can be applied for this class of problems. LÄS MER
3. Impact of glucose uptake rate on recombinant protein production in Escherichia coli
Sammanfattning : Escherichia coli (E. coli) is an attractive host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve high product quantities. LÄS MER
4. Modelling and control of microbial fed-batch and pH-auxostat processes
Sammanfattning : Glucose and energy metabolism ofSaccharomyces cerevisiaehas been studied for decades,yet the exact location of the so called "bottleneck" thatcauses overflow metabolism resulting in aerobic ethanolformation remains unknown. However, this does not completelyhamper the modelling, simulation and development of methods tocontrol the aerobic ethanol formation under processconditions. LÄS MER
5. Feeding Strategies Based on Probing Control for E. coli and V. cholerae Cultivations
Sammanfattning : The recombinant DNA techniques have made it possible to produce many different proteins for a wide range of applications. The vector encoding for the recombinant protein is often inserted in the bacterium E. coli as it is a well studied and a well-known organism. To achieve a high productivity, it is important to reach a high cell density. LÄS MER