Sökning: "enzyme design"

Visar resultat 6 - 10 av 218 avhandlingar innehållade orden enzyme design.

  1. 6. Exploiting enzyme promiscuity for rational design

    Författare :Cecilia Branneby; Karl Hult; Alex Zaks; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; Candida antarctica lipase B; alanine racemase; substrate specificity; reaction specificity; enzyme catalytic promiscuity; Biokemi; Biochemistry; Biokemi;

    Sammanfattning : Enzymes are today well recognized in various industrial applications, being an important component in detergents, and catalysts in the production of agrochemicals, foods, pharmaceuticals, and fine chemicals. Their large use is mainly due to their high selectivity and environmental advantage, compared to traditional catalysts. LÄS MER

  3. 7. Engineering Candida antarctica Lipase A for Enantioselective Transformations in Organic Synthesis : Design, Immobilization and Organic Solvent Screening of Smart Enzyme Libraries

    Författare :Ylva Wikmark; Jan-Erling Bäckvall; Romas Kazlauskas; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Candida antarctica Lipase A; protein engineering; enzyme libraries; enzyme immobilization; biocatalysis; organisk kemi; Organic Chemistry;

    Sammanfattning : The use of enzymes as catalysts in organic synthesis constitutes an attractive alternative to conventional chemical catalysis. Enzymes are non-toxic and biodegradable and they can operate under mild reaction conditions. LÄS MER

  4. 8. Amine Transaminases in Biocatalytic Amine Synthesis

    Författare :Henrik Land; Per Berglund; Helen C. Hailes; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Amine Transaminase; Biocatalysis; Transamination; Reductive Amination; Enzyme; Enzyme Engineering; Equilibrium Displacement; Screening; Enzyme Stability; Bioteknologi; Biotechnology;

    Sammanfattning : The use of enzymes, nature´s own catalysts, both isolated or as whole cells to perform chemical transformations is called biocatalysis. As a complement to classical chemical catalysis, biocatalysis can be an environmentally friendly and more economical option in the production and synthesis of chemicals. LÄS MER

  5. 9. Molecular Simulation of Enzyme Catalysis and Inhibition

    Författare :Sinisa Bjelic; Johan Åqvist; Adrian J. Mulholland; Uppsala universitet; []
    Nyckelord :Theoretical chemistry; enzyme catalysis; enzyme inhibition; computer simulations; molecular dynamics; empirical valence bond method; structure-based inhibitor design; Teoretisk kemi;

    Sammanfattning : The reaction mechanisms for the hemoglobin degrading enzymes in the Plasmodium falciparum malaria parasite, plasmepsin II (Plm II) and histo-aspartic protease (HAP), have been analyzed by molecular simulations. The reaction free energy profiles, calculated by the empirical valence bond (EVB) method in combination with molecular dynamics (MD) and free energy perturbation (FEP) simulations are in good agreement with experimental data. LÄS MER

  7. 10. Enzyme assays for lignin–carbohydrate bond hydrolysis

    Författare :Hampus Sunner; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Enzyme kinetic param- eter estimation; Glucuronoyl esterase; Enzyme assays; Lignin-carbohydrate bonds; Synergistic enzyme assay;

    Sammanfattning : To identify, produce, and use enzymes, analytical methods known as enzyme assays are employed. Enzyme assays are based on analysing the changes brought about on a substrate by an enzyme under defined conditions. Assays are often based on simplified reactions acting as substitutes for the reaction of interest. LÄS MER