Sökning: "dna probe"

Visar resultat 31 - 35 av 121 avhandlingar innehållade orden dna probe.

  1. 31. Padlock Probes and Rolling Circle Amplification : New Possibilities for Sensitive Gene Detection

    Författare :Maritha Mendel-Hartvig; Pål Nyrén; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Padlock probes; RCR; in situ hybridization; branched DNA; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

    Sammanfattning : A series of novel methods for detection of known sequence variants in DNA, in particular single nucleotide polymorphism, using padlock probes and rolling circle replication are presented. DNA probes that can be circularized – padlock probes – are ideal for rolling circle replication. LÄS MER

  3. 32. Biomolecular Analysis by Dual-Tag Microarrays and Single Molecule Amplification

    Författare :Olle Ericsson; Ulf Landegren; Mats Nilsson; Edwin Southern; Uppsala universitet; []
    Nyckelord :Molecular medicine; Microarray; proximity ligation; Padlock probe; Rolling circle amplification; Splicing; single molecule; Molekylärmedicin;

    Sammanfattning : Padlock probes and proximity ligation are two powerful molecular tools for detection of nucleic acids and proteins, respectively. Both methods result in the formation of DNA reporter molecules upon recognition of specific target molecules. LÄS MER

  4. 33. Biomolecular Recognition Based on Field Induced Magnetic Bead Dynamics

    Författare :Rebecca Stjernberg Bejhed; Peter Svedlindh; Klas Gunnarsson; Maria Strömme; Teresa Zardán Gómez de la Torre; Christer Johansson; Uppsala universitet; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Magnetic biosensor; magnetic nanoparticle; DNA detection; Teknisk fysik; Engineering Science;

    Sammanfattning : In this thesis, three different read-out techniques for biomolecular recognition have been studied. All three techniques rely on the change in dynamic behaviour of probe functionalised magnetic beads after binding to a biomolecular target complementary to the probe. LÄS MER

  5. 34. Pre-PCR Processing Strategies for Quantitative Detection of Food-Borne Pathogens using Real-Time PCR

    Författare :Petra Wolffs; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Livsmedelsteknik; Food and drink technology; DNA polymerase; sample treatment; pre-PCR processing; real-time PCR; Food-borne pathogens;

    Sammanfattning : Since the development of real-time PCR, the technology has been widely applied in the field of diagnostics. In comparison with conventional PCR it has opened up the possibility of accurate quantification of microorganisms in clinical, environmental and food samples. LÄS MER

  7. 35. Tagging systems for sequencing large cohorts

    Författare :Mårten Neiman; Afshin Ahmadian; Peter Savolainen; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; next generation sequencing; genotyping; massive parallel sequencing; 454; Pyrosequencing; amplicon sequencing; enrichment; DNA barcodes; Genetics; Genetik;

    Sammanfattning : Advances in sequencing technologies constantly improves the throughput andaccuracy of sequencing instruments. Together with this development comes newdemands and opportunities to fully take advantage of the massive amounts of dataproduced within a sequence run. LÄS MER