Sökning: "real-time PCR"

Visar resultat 1 - 5 av 271 avhandlingar innehållade orden real-time PCR.

  1. 1. Real-time PCR and its application for protein quantification

    Författare :Kristina Lind; [2007]
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; ELISA; real-time immuno-PCR; real-time PCR; melt curve analysis; PSA; 3’UTR; TAP-tag; BOXTO; immuno-qPCR;

    Sammanfattning : The ability to quantify specific DNA sequences with real-time PCR has increased the possibilities for sensitive DNA analysis enormously. This sensitive technique can in optimal conditions detect one DNA copy and has a large quantification range. LÄS MER

  2. 2. Kinetics Quality Assessment for real-time PCR

    Författare :Tzachi Bar; [2005]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; kinetics quality assessment; PCR quality assessment; outlier detection; real-time PCR;

    Sammanfattning : For proper DNA quantification by real-time PCR, compared samples should have similar PCR efficiencies. This assumption might not be always correct, as suggested by hundreds of publications dealing with PCR inhibition. LÄS MER

  3. 3. Gene expression profiling with real-time PCR

    Författare :Anders Ståhlberg; [2005]
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; single cell biology; reverse transcription; LightUp probe; cancer diagnostics; real-time PCR; gene expression profiling; tumor biology;

    Sammanfattning : The sequence of the entire human genome is available today, and this information can be used to understand, characterize, and treat complex diseases such as cancer. Many cellular functions related to survival, growth and differentiation are reflected in the gene expression pattern, and the ability to quantify transcription levels of specific genes is, and always has been, central to research on gene function. LÄS MER

  4. 4. Sensitive Forensic DNA Analysis Application of Pyrosequencing and Real-time PCR Quantification

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Hanna Andréasson; Uppsala universitet.; [2005]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; forensic; sensitive DNA analysis; DNA quantification; mtDNA; Real-time PCR; Pyrosequencing; Genetik; MEDICINE Dermatology and venerology; clinical genetics; internal medicine Clinical genetics; MEDICIN Dermatologi och venerologi; klinisk genetik; invärtesmedicin Klinisk genetik;

    Sammanfattning : The field of forensic genetics is growing fast and the development and optimisation of more sensitive, faster and more discriminating forensic DNA analysis methods is highly important. In this thesis, an evaluation of the use of novel DNA technologies and the development of specific applications for use in forensic casework investigations are presented. LÄS MER

  5. 5. Development of Real-Time PCR Based Methods for Detection of Viruses and Virus Antibodies

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Amal Elfaitouri; Uppsala universitet.; [2006]
    Nyckelord :Microbiology; Real-time PCR; broadly targeted PCR; degenerated PCR; rTth DNA polymerase; thermolabile UNG; QPIA; IgM; Mikrobiologi;

    Sammanfattning : Quantitative real-time PCR (QPCR) technology has been very useful for diagnosis of viral diseases. QPCR has recently reached a level of sensitivity, simplicity, and reproducibility which allows a large number of samples to be screened rapidly, make it a suitable tool for the clinical virology diagnostics. LÄS MER