Sökning: "catalytic promiscuity"

Visar resultat 6 - 10 av 12 avhandlingar innehållade orden catalytic promiscuity.

1. 6. Chemoenzymatic Resolution in Dynamic Systems : Screening, Classification and Asymmetric Synthesis

   Författare :Yan Zhang; Olof Ramström; Sabine Flitsch; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; constitutional dynamic chemistry; dynamic systemic resolution; dynamic kinetic resolution; enzyme catalysis; transesterification; enzyme promiscuity; asymmetric synthesis; pattern recognition; self-inhibition.;

   Sammanfattning : This  thesis  is  divided  into  four  parts,  all  centered  around  Constitutional Dynamic  Chemistry  (CDC)  and  Dynamic  Kinetic  Resolution  (DKR)  using biocatalysts for selective transformations, and their applications in screening of bioactive compounds, organic synthesis, and enzyme classification.   In  part  one,  an  introduction  to  CDC  and  DKR  is  presented,  illustrating  the basic  concepts,  practical  considerations  and  potential  applications  of  such dynamic systems, thus providing the background information for the studies in the following chapters. LÄS MER

3. 7. Directed Evolution of Glutathione Transferases with Altered Substrate Selectivity Profiles : A Laboratory Evolution Study Shedding Light on the Multidimensional Nature of Epistasis

   Författare :Wei Zhang; Bengt Mannervik; Bengt-Harald Jonsson; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; glutathione transferase; azathioprine; directed evolution; semi-rational design; catalytic mechanism; saturation mutagenesis; reduced amino acid alphabets; molecular docking; protein evolution; multivariate data analysis; epistasis; fitness landscape; evolutionary trajectories; Biochemistry; Biokemi; Biokemi; Biochemistry;

   Sammanfattning : Directed evolution is generally regarded as a useful approach in protein engineering. By subjecting members of a mutant library to the power of Darwinian evolution, desired protein properties are obtained. Numerous reports have appeared in the literature showing the success of tailoring proteins for various applications by this method. LÄS MER

4. 8. Engineered Alcohol Dehydrogenases for Stereoselective Chemical Transformations

   Författare :Thilak Reddy Enugala; Mikael Widersten; Manfred T. Reetz; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; alcohol dehydrogenase-A; biocatalysts; protein engineering; enzyme kinetics; sec-alcohols; ketones; stereoselectivity; regioselectivity; substrate selectivity and promiscuity.; Biokemi; Biochemistry;

   Sammanfattning : Enzymes are biomolecules built from amino acids and catalyze the chemical transformations in a cell. Enzymes are by nature stereoselective, biodegradable, environmentally friendly, and can perform catalysis in aqueous solutions and at ambient temperatures. LÄS MER

5. 9. Exploring Conjugate Addition Activity in Pseudozyma antarctica Lipase B

   Författare :Maria Svedendahl; Per Berglund; Olof Ramström; Amelie Eriksson Karlström; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Candida antarctica lipase B · conjugate addition · enzyme catalysis · enzyme catalytic promiscuity · molecular modeling; Biochemistry; Biokemi;

   Sammanfattning : Multifunctional enzymes have alternative functions or activities, known as “moonlighting” or “promiscuous”, which are often hidden behind a native enzyme activity and therefore only visible under special environmental conditions. In this thesis, the active-site of Pseudozyma (formerly Candida) antarctica lipase B was explored for a promiscuous conjugate addition activity. LÄS MER

7. 10. Rational engineering of esterases for improved amidase specificity in amide synthesis and hydrolysis

   Författare :Peter Hendil-Forssell; Mats Martinelle; Romas J. Kazlauskas; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Amidase; Biocatalysis; Enzyme; Esterase; Enzyme engineering; Lipase; Substrate specificity; Bioteknologi; Biotechnology;

   Sammanfattning : Biocatalysis is an ever evolving field that uses enzymes or microorganisms for chemical synthesis. By utilizing enzymes that generally have evolved for specific reactions under mild conditions and temperatures, biocatalysis can be a more environmentally friendly option compared to traditional chemistry. LÄS MER